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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia
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Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia

机译：安纳托利亚中部鹰嘴豆疫病的致病病原体狂犬病Ascochyta rabiei（Teleomorph：Didymella rabiei）的交配类型群

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摘要

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 (57.8%) and the others (42.2%) to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods.
机译：研究了从安那托利亚中部鹰嘴豆生长地区收集的分离株的交配类型Ascochyta rabiei（teleomorph：Didymella rabiei）的分布。在实验室条件下对来自6个不同省份的45个分离株的交配类型进行了评估。保持5-6周后，在8±1°C和85％相对湿度下温育的鹰嘴豆茎片上观察到假神经发育和子囊孢子产生。除开塞利和锡瓦斯外，在所有省份都发现了两种交配类型。大多数分离株属于Mat 1-1（57.8％），其他（42.2％）属于MAT 1-2。此外，使用引物Com1，Tail 5和SP21的多重PCR方法将狂犬病菌的交配类型区分开。根据常规方法对交配类型组的区分，将MAT 1-1分离株扩增为约460 bp片段，将MAT 1-2分离株扩增为700 bp片段。

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