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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >The Effects of Shoot Tip Source on the Success of In Vitro Micrografting in Grapevine (Vitis vinifera L.)
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The Effects of Shoot Tip Source on the Success of In Vitro Micrografting in Grapevine (Vitis vinifera L.)

机译：茎尖来源对葡萄（Vitis vinifera L.）体外微移植成功的影响

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摘要

In this experiment, the effects of the shoot tip source on the success of in vitro micrografting in grapevine were investigated. Shoot tip meristems of Kalecik karas?, Emir, Uslu, Haf?zali and Razak? grape cultivars were micrografted onto the seedlings of Kober 5 BB. Shoot tip meristems were excised from either in vivo (shoots from actively growing vine in the vineyard and shoots flushed from one year-old cuttings in the greenhouse) or in vitro (shoots obtained by shoot-tip culture in aseptic conditions) sources. Plantlets micrografted on top of the decapitated seedlings were cultured in solid MS medium for two months. Consequently, it was determined that the success of micrografting was affected by the shoot tip source and the highest values (40.9-68.2 %) were obtained from in vitro shoot tips. It was also observed that the shoot tip sources had no marked effect on the shoot and or development of the micrografted plants, although the success of grafting was influenced by shoot tip sources. Furthermore, the success of the micrografting varied between the cultivars.
机译：在该实验中，研究了茎尖来源对葡萄体外显微嫁接成功的影响。拍摄Kalecik karas？，Emir，Uslu，Haf？zali和Razak？的尖端分生组织。将葡萄品种微移植到Kober 5 BB的幼苗上。从体内（从活跃生长的葡萄藤中收获的枝条和从温室中一岁的插条中冲出的枝条）或体外（在无菌条件下通过枝尖培养获得的枝条）来源切除枝条分生组织。微植在断头的幼苗顶部的小植株在固体MS培养基中培养两个月。因此，确定了微嫁接的成功受到枝梢来源的影响，并且从体外枝梢获得了最高值（40.9-68.2％）。还观察到，尽管嫁接的成功受到芽尖来源的影响，但芽尖来源对微嫁接植物的芽和/或发育没有显着影响。此外，微嫁接的成功因品种而异。

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