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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >High Efficiency Somatic Embryogenesis from Immature Zygotic Embryos of Grapevine: The Effect of Genotype, Media, 2,4-D, and Incubation Conditions
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High Efficiency Somatic Embryogenesis from Immature Zygotic Embryos of Grapevine: The Effect of Genotype, Media, 2,4-D, and Incubation Conditions

机译：葡萄未成熟合子胚的高效体细胞胚发生：基因型，培养基，2，4-D和培养条件的影响

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Immature zygotic embryos of 41 B grape rootstock (V. vinifera L. cv. 'Chasselas' x V. berlandieri) and Yalova ?ncisi (V. vinifera L.) were cultured on MS, NN, and B5 media supplemented with 0.5, 1, 2, and 4 mg l-1 2,4-D at 16/8 h light/dark photoperiod and complete darkness. For 41 B rootstock, the highest somatic embryo formation was obtained from dark condition on B5 media containing 0.5 and 1 mg l-1 2,4-D with the rates of 30% and 28.9%, respectively. Yalova ?ncisi zygotic embryos produced 5% somatic embryos only at 16/8 h light/dark photoperiod of MS supplemented with 1 mg l-1 2,4-D, and also in 16/8 h light/dark photoperiod and complete darkness somatic embryos were produced at the rates of 6.3% and 2.3%, respectively, in cultures of NN containing 0.5 mg l-1 2,4-D. After 8 months of culture, 559 embryos at torpedo stage were identified on B5 + 1 mg l-1 2,4-D + dark cultures of 41 B and 912 embryos at torpedo stage were identified on NN + 0.5 mg l-1 2,4-D + light cultures of Yalova ?ncisi. The highest germination and plantlet conversion rates were obtained from 41 B on free NN medium (58% and 75%, respectively) and from Yalova ?ncisi on free MS medium (77.4% and 45%, respectively). Of the regenerated somatic plantlets, 91.9% were successfully transferred to soil.
机译：在MS，NN和B5培养基中分别培养了41个B葡萄砧木（V. vinifera L. cv。'Chasselas'x V. berlandieri）和Yalova？ncisi（V. vinifera L.）的未成熟合子胚。 ，2和4 mg l-1 2,4-D在16/8 h光照/黑暗光周期和完全黑暗下。对于41 B砧木，在黑暗条件下，在含有0.5和1 mg l-1 2,4-D的B5培养基上，最高的体细胞胚形成率分别为30％和28.9％。 Yalova？ncisi合子胚仅在补充有1 mg l-1 2,4-D的MS的16/8 h光照/黑暗光周期以及16/8 h光照/黑暗光周期和完全黑暗的体细胞中产生5％的体细胞胚在含有0.5mg L-1的2，4-D的NN的培养物中，分别以6.3％和2.3％的速率产生胚。培养8个月后，在B5 + 1 mg l-1 2,4-D上鉴定出559个鱼雷期胚胎，在NN + 0.5 mg l-1 2上鉴定出41 B的深色培养物和912个在鱼雷期的胚胎。亚洛瓦·芬西斯的4-D +光文化。在游离NN培养基上分别从41 B（分别为58％和75％）和在游离MS培养基上从Yalova Finsis（分别为77.4％和45％）获得最高的发芽率和幼苗转化率。在再生的体细胞小植株中，成功地将91.9％转移到土壤中。

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