...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Drug Design, Development and Therapy >Integration of microRNA–mRNA profiles and pathway analysis of plant isoquinoline alkaloid berberine in SGC-7901 gastric cancers cells
【24h】

Integration of microRNA–mRNA profiles and pathway analysis of plant isoquinoline alkaloid berberine in SGC-7901 gastric cancers cells

机译：microGC–mRNA图谱的整合和植物异喹啉小碱在SGC-7901胃癌细胞中的通路分析

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Purpose: Berberine (BBR) is a traditional Chinese medicine normally used for gastroenteritis, and recent research found that it could fight against tumors. In this study, we focused on integrating miRNA sequencing and RNA sequencing of SGC-7901 gastric cancer cells treated by BBR to elucidate their underlying mechanisms. Materials and methods: WST-1 assay and flow cytometry were used to check the effects of BBR on SGC-7901. miRNA sequencing and RNA sequencing were used to establish the miRNA and mRNA profiles of BBR-treated SGC-7901. Results: The results showed that BBR could inhibit the proliferation of SGC-7901 cells and induce G1 arrest in cell cycle phase and apoptosis. A total of 1,960 upregulated genes and 4,837 downregulated genes were identified in the RNA sequencing and 347 upregulated and 93 downregulated miRNAs in the miRNA sequencing. A total of 78 novel miRNAs were also found. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that the genes were related to pathways in cancer and metabolism. We also analyzed the miRNA–mRNA network of genes grouped into cell cycle, apoptosis, inflammation, metabolism, cell junction, acetylization process, TGF-β pathway, and Wnt signaling pathway. Conclusion: BBR could inhibit the proliferation of SGC-7901 cells and induce apoptosis. Integrated analysis of microRNA–mRNA profiles is a promising approach to validate gene expression patterns associated with malignant phenotype and study the mechanisms of anticancer.
机译：目的：小ber碱（BBR）是一种通常用于胃肠炎的中药，最近的研究发现它可以对抗肿瘤。在这项研究中，我们集中于整合miRNA测序和BBR治疗的SGC-7901胃癌细胞的RNA测序，以阐明其潜在机制。材料与方法：采用WST-1测定法和流式细胞仪检查BBR对SGC-7901的作用。 miRNA测序和RNA测序用于建立BBR处理的SGC-7901的miRNA和mRNA谱。结果：结果表明，BBR可以抑制SGC-7901细胞的增殖，并诱导G1阻滞于细胞周期和凋亡。在RNA测序中共鉴定出1,960个上调基因和4,837个下调基因，在miRNA测序中鉴定出347个上调基因和93个下调基因。还发现了总共78种新颖的miRNA。基因本体论和京都基因与基因组百科全书分析表明，这些基因与癌症和代谢途径有关。我们还分析了miRNA–mRNA基因网络，这些基因分为细胞周期，凋亡，炎症，代谢，细胞连接，乙酰化过程，TGF-β途径和Wnt信号传导途径。结论：BBR可抑制SGC-7901细胞增殖并诱导其凋亡。 microRNA–mRNA谱图的综合分析是验证与恶性表型相关的基因表达模式并研究抗癌机制的一种有前途的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号