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Sequencing of COI gene in four rodent pests for species identification

机译:四种啮齿动物害虫COI基因的序列鉴定

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Among mammals, rodents are the most important group since they influence the crop production and protection. Species identification of rodents on morphometric parameters is cumbersome and often misleading. However, DNA based identification would enable accurate species identification. For this purpose, we examined 650 bp of Cytochrome c oxidase subunit1 (COI) in four rodent pest species namely Bandicota indica, Milardia meltada, Rattus rattus and Tatera indica. Our results show the maximum identity for Bandicota indica and Rattus rattus within the species. For T. indica the maximum similarity was observed with gerbillinae species and M. meltada showed the maximum identity with B. indica. The result indicates that COI based identification can serve as an accurate and reliable tool in the identification of rodent pest species.
机译:在哺乳动物中,啮齿动物是最重要的群体,因为它们影响农作物的生产和保护。根据形态学参数对啮齿动物进行物种鉴定很麻烦,而且常常会产生误导。但是,基于DNA的识别将能够进行准确的物种识别。为此,我们检查了四种啮齿动物害虫,即印度班迪卡塔州,米尔拉迪亚蜜达达,褐家鼠和印度泰特拉中的650 bp细胞色素C氧化酶亚基1(COI)。我们的结果显示了该物种中印度Band和褐家鼠的最大身份。对于印度T,与沙鼠科物种具有最大的相似性,而M.melada与印度B具有最大的相似性。结果表明,基于COI的鉴定可作为鉴定啮齿动物害虫种类的准确可靠的工具。

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