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首页> 外文期刊>Japanese Journal of Pharmacology >Effect of Saiboku-to, an Antiasthmatic Herbal Medicine, on Nitric Oxide Generation from Cultured Canine Airway Epithelial Cells
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Effect of Saiboku-to, an Antiasthmatic Herbal Medicine, on Nitric Oxide Generation from Cultured Canine Airway Epithelial Cells

机译：Saiboku-to（一种抗哮喘药）对培养的犬气道上皮细胞产生一氧化氮的影响

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References(40) Cited-By(4) The effect of Saiboku-to (TJ-96), an antiasthmatic Kampo medicine, on the generation of nitric oxide (NO) from cultured canine tracheal epithelium was investigated using a highly specific amperometric sensor for this molecule in vitro. Immersion of the NO-selective electrode in the medium containing tracheal epithelial cells detected the baseline current of 16.8 - 57.0 pA, which corresponded to an NO concentration ([NO]) of 39.7 ± 8.1 nM. Addition of TJ-96 increased [NO] in a concentration-dependent manner, the maximal increase from the baseline level and the concentration of TJ-96 required to produce a half-maximal effect (EC50) being 127.5 ± 20.1 nM (P 0.001) and 86 ± 9 μg/ml, respectively. Pretreatment of cells with NG-nitro-L-arginine methylester (L-NAME) greatly inhibited the TJ-96-induced increase in [NO], whereas NG-nitro-D-arginine methylester (D-NAME) had no effect, and this inhibition was reversed by L-arginine but not by D-arginine. Cytochemical staining of the epithelial cells showed marked reactivity of NADPH diaphorase activity. These results suggest that NO is spontaneously released by the airway epithelium and that TJ-96 stimulates the epithelial NO generation.
机译：参考文献（40）被引用的文献（4）使用高度特异性的安培传感器研究了Saiboku-to（TJ-96），一种抗哮喘的Kampo药物，对培养的犬气管上皮产生一氧化氮（NO）的影响。这个分子在体外。将NO选择电极浸入含有气管上皮细胞的培养基中，可以检测到16.8-57.0 pA的基线电流，这对应于39.7±8.1 nM的NO浓度（[NO]）。 TJ-96的添加以浓度依赖的方式增加[NO]，相对于基线水平的最大增加以及产生半最大作用（EC50）所需的TJ-96的浓度为127.5±20.1 nM（P <0.001 ）和86±9μg/ ml。用NG-硝基-L-精氨酸甲酯（L-NAME）预处理细胞会大大抑制TJ-96诱导的[NO]增加，而NG-硝基-D-精氨酸甲酯（D-NAME）没有作用，并且L-精氨酸逆转了这种抑制作用，而D-精氨酸逆转了这种抑制作用。上皮细胞的细胞化学染色显示出NADPH心肌黄递酶活性的显着反应性。这些结果表明，NO由气道上皮自发释放，并且TJ-96刺激上皮NO的产生。

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