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首页> 外文期刊>Journal of Advanced Science >生体適合性高分子基板を用いたフレキシブルバイオセンサ
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生体適合性高分子基板を用いたフレキシブルバイオセンサ

机译：使用生物相容性聚合物基质的柔性生物传感器

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In this study, a flexible glucose sensor with biocompatible polymer substrate for biomedical measurement was constructed and tested. The sensor was constructed by immobilizing an enzyme to biocompatible polymer substrate with electrodes. In the fabrication of the sensor, Pt-working and Ag/AgCl-counter/reference electrodes were formed onto a flexible polymer substrate by MEMS technique. In the immobilization process of an enzyme, a mixture solution of glucose oxidase (GOD) and PMEH as a biocompatible polymer was spread onto the sensing region of the electrodes, thus obtaining the flexible glucose biosensor with biocompatibility. In the evaluation of the sensor characteristics, the sensor was dipped into a measurement cell filled phosphate buffer, and then a +400mV potential was applied to Pt-working electrode vs. Ag/AgCl-counter/reference electrode by potentiostat controlled by PC. Various concentrations of glucose solution were dropped into the beaker and the output current of the sensor was measured by PC via A/D converter. As a result of characteristics evaluation, the output current rapidly increased immediately after the drop of glucose solution and linearly corresponded to glucose concentration over a range of 0.05-4.0mmol/l, with a correlation coefficient of 0.999. The result suggests that GOD was successfully immobilized onto the substrate. This sensor would be applied to detecting tear glucose of rabbit for continuous evaluation of blood sugar level in the near future.
机译：在本研究中，构建并测试了具有生物相容性聚合物底物的柔性葡萄糖传感器，用于生物医学测量。通过将酶固定到带有电极的生物相容性聚合物底物上来构建传感器。在传感器的制造中，通过MEMS技术将Pt工作电极和Ag / AgCl反电极/参比电极形成在柔性聚合物基板上。在酶的固定过程中，将作为生物相容性聚合物的葡萄糖氧化酶（GOD）和PMEH的混合溶液铺展在电极的传感区域上，从而获得具有生物相容性的柔性葡萄糖生物传感器。在评估传感器特性时，将传感器浸入装有磷酸钙的测量池中，然后通过PC控制的恒电位仪将+ 400mV电势施加到Pt工作电极与Ag / AgCl-反电极/参比电极上。将各种浓度的葡萄糖溶液滴入烧杯中，并通过PC通过A / D转换器测量传感器的输出电流。作为特性评价的结果，在葡萄糖溶液滴加之后，输出电流迅速增加，并且在0.05-4.0mmol / l的范围内线性对应于葡萄糖浓度，相关系数为0.999。结果表明，GOD已成功固定在基质上。该传感器将被用于检测兔子的泪液葡萄糖，以在不久的将来连续评估血糖水平。

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