• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Advanced Science >Isolation of repetitive sequence elements from the genome of a mayfly Rhithrogena japonica (Ephemeroptera: Heptageniidae)
【24h】

Isolation of repetitive sequence elements from the genome of a mayfly Rhithrogena japonica (Ephemeroptera: Heptageniidae)

机译：从may蝇Rhithrogena japonica（Ephemeroptera：Heptageniidae）的基因组中分离重复序列元件。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Isolation of short, tandem repeat sequences from mayfly Rhithrogena japonica Uéno 1928 has been carried out by probing the multicopy DNA regions using labeled total genomic DNA. Several microsatellites comprised of pyrimidine/purine-biased (Pyr/Pur) repeating units (CT, TCTG, and TCTCTG) and two minisatellites were identified. No locus containing (CA)_(n) repeat has been identified so far by screening with total genomic DNA and (CA)_(25) probes. The Pur/Pyr microsatellites may be more abundant than (CA)_(n) microsatellite in the R. japonica genome. Both minisatellite sequences, termed Rja66 family (107bp repeating unit) and Taq I-family (repeating unit about 160bp), have self-complementary regions within the repeating units, thereby conforming to stem-loop structures in single-stranded DNA. As for microsatellite loci, a preliminary analysis on two microsatellites Rja18-1 (CT repeat, FJ827088) and Rja130F (TCTCTG repeat, FJ826512) was done for a local population of R. japonica , showing that they were highly polymorphic. Characterization of genetic structures of local R. japonica populations using Rja18-1 locus, demonstrated significant differences between geographically distant populations indicating an impaired genetic flow between such populations.
机译：通过使用标记的总基因组DNA探测多拷贝DNA区域，可以从may蝇Rhithrogena japonicaUéno1928中分离出短串联重复序列。鉴定了由嘧啶/嘌呤偏置的（Pyr / Pur）重复单元（CT，TCTG和TCTCTG）组成的几个微卫星和两个微型卫星。到目前为止，尚未通过总基因组DNA和（CA）_（25）探针的筛选来确定含有（CA）_（n）重复序列的基因座。在日本粳稻基因组中，Pur / Pyr微卫星可能比（CA）_（n）微卫星更为丰富。称为Rja66家族（107bp重复单元）和Taq I家族（约160bp重复单元）的小卫星序列在重复单元内均具有自互补区，从而符合单链DNA中的茎环结构。对于微卫星基因座，对两个Rja18-1（CT重复，FJ827088）和Rja130F（TCTCTG重复，FJ826512）微卫星进行了初步分析，表明它们具有很高的多态性。使用Rja18-1基因座表征日本粳稻种群的遗传结构，表明地理上远距离的种群之间存在显着差异，表明这些种群之间的遗传流通受到损害。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号