Plasmid Profile Analysis of Phage Sensitive E.coli Strains from Human Sewage System

Emel Karadeniz; Fatma Esen Sarigullu; Isil Untac; Omer Colak

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Plasmid Profile Analysis of Phage Sensitive E.coli Strains from Human Sewage System

机译：人类污水系统中噬菌体敏感大肠杆菌的质粒谱分析

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Abstract In this study, animal and human pathogen Escherichia coli strains were isolated from Balcaly domestic sewage water. The isolated strains were identified and 37 strains which are sensitive to phage were selected. The plasmid isolation of bacteria strains were done and plasmid profiles were observed by agarose gel electrophoresis. Except some strains, most of the E.coli strains had 300-10,000 bp sized plasmids and also greater than 10,000 bp sized plasmids were determined. With our research, we aim to provide contribution that the pathogenic E.coli strains can be eliminated naturally by lysing with their specific phages in any environment.

机译：摘要在本研究中，从Balcaly生活污水中分离出动物和人类病原体大肠杆菌菌株。鉴定出分离的菌株并选择对噬菌体敏感的37个菌株。完成细菌菌株的质粒分离，并通过琼脂糖凝胶电泳观察质粒图谱。除某些菌株外，大多数大肠杆菌菌株均具有300-10,000 bp大小的质粒，并且还确定了大于10,000 bp大小的质粒。通过我们的研究，我们旨在为通过在任何环境中溶解特定的噬菌体而自然消除病原性大肠杆菌作出贡献。

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《Journal of Animal Science Advances》 |2012年第10期|共6页
作者
Emel Karadeniz; Fatma Esen Sarigullu; Isil Untac; Omer Colak;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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2. Characterization of Phage-Sensitive Mutants from a Phage-Insensitive Strain of Streptococcus lactis: Evidence for a Plasmid Determinant that Prevents Phage Adsorption [J] . Mary Ellen Sanders, Todd R. Klaenhammer Applied and Environmental Microbiology . 1983,第5期

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6. Differentiation of Salmonella enteritidis phage type 8 strains: evaluation of three additional phage typing systems plasmid profiles antibiotic susceptibility patterns and biotyping. [O] . A D Stubbs, F W Hickman-Brenner, D N Cameron, 1994

机译：肠炎沙门氏菌噬菌体8型菌株的分化：评估另外三个噬菌体分型系统质粒图谱抗生素敏感性模式和生物分型。
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机译：Salmonella Enteritidis噬菌体型8株的分化：评价三种附加噬菌体键入系统，质粒谱，抗生素敏感模式和生物型

Plasmid Profile Analysis of Phage Sensitive E.coli Strains from Human Sewage System

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