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首页> 外文期刊>Journal of Applied Animal Research >Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae
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Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae

机译：荆芥提取物用于控制蜜蜂致病菌和蚊虫幼虫的用途

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ABSTRACT Honeybee ( Apis mellifera ) population is going down across the globe due to honeybee pathogens. This greatly influences the bee-associated commercial food products production. Likewise, mosquitoes are prominent vector responsible for spreading life-threatening human diseases, including malaria and dengue. The plant-based insecticides are a better substitute to the recent control practices of honeybee pathogenic bacteria and mosquito. Here, we performed in vitro screening of Nepeta clarkei Hook.????f.????(Labiatae) aqueous extracts against three honey bee gut bacterial isolates including Paenibacillus larvae an infamous honeybee bacterial pathogen. The inhibitory zone was produced in the range of 6?￠????14?￠????mm diameters against three honey bee bacterial isolates . Likewise, fourth instars larvae of Culex (Diptera/Culicidae) were also subjected to check the possible larvicidal efficacy of N. clarkei . A normal media supplemented with N. clarkei in different concentrations (0.025% 0.05%, 0.1%, 0.15%, and 0.2%) affected the growth of larvae significantly. The lethal concentration at which 50% of larvae failed to become pupate was found to be 0.1% after 24?￠????h of exposure. Considerable reductions in larval growth and pupal development of mosquito suggested that this plant should be utilized in mosquito control programmes.
机译：摘要蜜蜂（Apis mellifera）的种群因蜜蜂病原体而在全球范围内减少。这极大地影响了与蜂有关的商业食品的生产。同样，蚊子是导致疟疾和登革热等威胁生命的人类疾病传播的重要媒介。以植物为基础的杀虫剂可以更好地替代蜜蜂病原细菌和蚊子的最新防治方法。在这里，我们针对三株蜜蜂肠道细菌分离株，包括臭名昭著的蜜蜂细菌病原体，对荆芥双歧（Labiatae）水提取物进行了体外筛选。相对于三种蜜蜂细菌分离物，抑制区的直径范围为6mm×14mm×14mm。同样，库克斯（Diptera / Culicidae）的四龄幼虫也要接受克拉克猪笼草的杀幼虫功效。补充了不同浓度（0.025％0.05％，0.1％，0.15％和0.2％）的克拉克猪笼草的正常培养基会显着影响幼虫的生长。暴露24小时后，发现50％的幼虫不能变成化ate的致死浓度为0.1％。蚊子幼虫生长和幼虫发育的明显减少表明该植物应用于蚊子控制计划中。

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