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首页> 外文期刊>Journal of Agricultural Biotechnology and Sustainable Development >Genetic diversity of chickpea (Cicer arietinum L.) cultivars from Ethiopia by using ISSR markers
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Genetic diversity of chickpea (Cicer arietinum L.) cultivars from Ethiopia by using ISSR markers

机译：利用ISSR标记研究埃塞俄比亚鹰嘴豆（Cicer arietinum L.）品种的遗传多样性。

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Inter Simple Sequence Repeat (ISSR) markers were employed to reveal genetic diversity and relatedness among 27 chickpea (Cicer arietinum L.) cultivars in Ethiopia. Four di-nucleotide repeat primers amplified 24 clear and reproducible bands of which 22 were polymorphic (91.67%). The genetic variation among 27 chickpea cultivars including 12 kabuli and 15 desi verities is high; in which desi type exhibited a genetic diversity of 75% with Shannon index of 0.47, while the kabuli type chickpea had 91.67% genetic diversity and Shannon index of 0.50. Unweighted Pair Group of Arithmetic mean (UPGMA) dendrogram and NJ trees with Jaccard’s similarity coefficient showed three major clusters. This was also recovered by 3D principal coordinates analysis, although some cultivars were intermixed. Analysis of molecular variance (AMOVA) demonstrated highly significant (p < 0.001) genetic diversity within cultivars (97.71%) than among cultivars (2.29%). The distinct cultivars (Aererti, Tejie, Fetenech and Maryie) can serve as parents for future genetic resources conservation and Chickpea breeding program in Ethiopia.Key word: Chickpea cultivars, genetic diversity, ISSR markers, Ethiopia.
机译：使用简单序列间重复（ISSR）标记揭示埃塞俄比亚27个鹰嘴豆（Cicer arietinum L.）品种之间的遗传多样性和相关性。四个二核苷酸重复引物扩增了24条清晰可重复的条带，其中22条为多态性（91.67％）。 27个鹰嘴豆品种的遗传变异很高，包括12个喀布尔和15个理想种。其中desi型具有75％的遗传多样性，香农指数为0.47，而喀布尔式鹰嘴豆具有91.67％的遗传多样性，香农指数为0.50。具有Jaccard相似系数的算术均值（UPGMA）树状图和NJ树的非加权对组显示了三个主要聚类。尽管有些品种混合在一起，但也可以通过3D主坐标分析将其恢复。分子变异分析（AMOVA）显示，品种内（97.71％）的遗传多样性高于品种间（2.29％）的显着（p <0.001）遗传多样性。不同的品种（Aererti，Tejie，Fetenech和Maryie）可以作为埃塞俄比亚未来遗传资源保护和鹰嘴豆育种计划的父母。关键词：鹰嘴豆品种，遗传多样性，ISSR标记，埃塞俄比亚。

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