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首页> 外文期刊>Journal of Behavioral and Brain Science >Design and Development of a High-Throughput System for Learning and Memory Research on Zebrafish
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Design and Development of a High-Throughput System for Learning and Memory Research on Zebrafish

机译：斑马鱼学习与记忆研究高通量系统的设计与开发

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Background: Since 2004, zebrafish have become the state-of-the-art, in vivo model for biomedical research due to their genetic and physiological homology with humans, inexpensive high-quantity breeding, and quick development in a highly-controlled environment suitable for longitudinal studies. New Method: To fully utilize the zebrafish model, a novel, automated, high-throughput system was designed. Shoals of five zebrafish were placed in 16 tanks and automatically fed over two days for a total of 16 training sessions. Color LED lights were used as the stimulus for each shoal coinciding with the release of food for a duration of 20 seconds. This system was tested on two age groups: 6- and 11-month-old. Results: After three training sessions, the median height of the school in the tank during stimulus was significantly higher than that of the na ï ve fish during the first training session. All subsequent training sessions demonstrated similar behaviour. A decline in memory retention, as defined by a reduction in the median height during light stimulus (i.e. no simultaneous food delivery), was observed 8 days post training. Comparison with existing methods: The high-throughput nature of this system allows for simultaneous training of 16 tanks of fish under identical conditions without human interaction and provides a means to rapidly assess their learning and memory behaviours. Conclusion: Results provide a baseline for understanding the normal cognitive processes of learning and memory retention in zebrafish. This work paves the way for future studies on the impacts of therapeutic agents on these cognitive processes.
机译：背景：自2004年以来，由于斑马鱼与人类之间的遗传和生理同源性，廉价的高品质育种以及在动物体内的快速发展，它们已成为生物医学研究的最新体内模型。高度受控的环境，适合进行纵向研究。 新方法：为了充分利用斑马鱼模型，设计了一种新颖的自动化高通量系统。将五个斑马鱼的浅滩放置在16个水箱中，并在两天内自动喂食，总共进行了16次训练。彩色LED灯被用作每个浅滩的刺激物，同时持续20秒的食物释放。该系统已在两个年龄组（6个月和11个月大）上进行了测试。 结果：经过三轮训练后，在刺激期间鱼缸中学校的平均身高显着高于第一轮训练中幼鱼的身高。随后的所有培训课程都表现出类似的行为。训练后8天观察到记忆保持力下降，这是由轻度刺激期间中位数身高的降低（即没有同时送餐）引起的。 与现有方法的比较：该系统的高通量特性允许在相同条件下同时训练16箱鱼，而无需人工干预，并提供了一种快速评估其学习和记忆行为的方法。 结论：结果为了解斑马鱼的正常学习和记忆保持的认知过程提供了基线。这项工作为将来关于治疗剂对这些认知过程影响的研究铺平了道路。

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