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首页> 外文期刊>Journal of Applied Life Sciences International >Effect of Chrysactinia mexicana Gray Extract on Laying Hens Organs Challenged with Salmonella typhimurium
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Effect of Chrysactinia mexicana Gray Extract on Laying Hens Organs Challenged with Salmonella typhimurium

机译：墨西哥乳壶菌灰色提取物对鼠伤寒沙门氏菌攻击的产蛋鸡器官的影响

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Introduction: Backyard poultry production systems are a very important source of meat and egg protein especially for children in rural areas. Aim: Evaluate the biocide effect of Chrysactinia mexicana Gray extract on laying hens organs challenged with Salmonella typhimurium. Study Design: In vitro and in vivo testing. Methodology: Two trials were conducted. In the first trial an in vitro experiment was performed with different solvents: aqueous, methylene chloride, ethanol and hexane for the plant extraction. Bactericide effect was determined. The second trial was an in vivo experiment 24 Plymouth Rock Barred hens, 21 weeks old (6 hens/treatment) were used with the following treatments: T1 control, no challenge and no plant extract; T2 control with challenge; T3 challenge and ethanol extract of C. mexicana and; T4 challenge and antibiotic. Colony Forming Units (CFU) of S. typhimurium in gizzard, ceca, crop and duodenum contents was measured. Results: Bactericide assessment of C. mexicana with different solvent extracts resulted effective against S. typhimurium on concentrations between 20 and 25 mg/ml of extract. Ethanol extract has higher bactericide activity. Feed intake, total weight gain and final body weight was higher for T1 among the other treatments. Treatment 2 had the lowest trait performance. T3 and T4 had similar feed intake, total weight gain and final body weight response. The control group had lower CFU for crop, gizzard, ceca and duodenum compared with the other treatments. The highest content of CFU for all four organs was for the T2. The treatment challenged with S. typhimurium and C. mexicana extract had lower CFU for the organs than T2. Treatment challenged and with antibiotic showed better CFU counts than T3. Conclusion: C. mexicana extract had a beneficial effect both in vitro and in vivo trials.
机译：简介：后院家禽生产系统是非常重要的肉和蛋蛋白来源，尤其是对于农村地区的儿童而言。目的：评估墨西哥乳壶菌灰色提取物对鼠伤寒沙门氏菌攻击的蛋鸡器官的杀生物作用。研究设计：体外和体内测试。方法：进行了两项试验。在第一个试验中，使用不同的溶剂进行了体外实验：用于植物提取的水溶液，二氯甲烷，乙醇和己烷。确定了杀菌作用。第二项试验是一项体内实验，使用21周大的24头普利茅斯罗克母鸡（6只母鸡/处理），进行以下处理：T1对照，无攻击，无植物提取物;挑战的T2控制; T3攻击和墨西哥锥虫的乙醇提取物;以及T4挑战和抗生素。测量了鼠伤寒沙门氏菌在g，盲肠，农作物和十二指肠中的菌落形成单位（CFU）。结果：用不同的溶剂提取物对墨西哥念珠菌进行杀菌评估，结果表明提取物浓度在20至25 mg / ml时对鼠伤寒沙门氏菌有效。乙醇提取物具有较高的杀菌活性。在其他治疗中，T1的采食量，总增重和最终体重较高。处理2的性状表现最低。 T3和T4具有相似的采食量，总增重和最终体重反应。与其他处理相比，对照组的作物、,、盲肠和十二指肠的CFU较低。所有四个器官的CFU含量最高的是T2。鼠伤寒沙门氏菌和墨西哥念珠菌提取物攻击的治疗器官的CFU低于T2。具有挑战性的治疗和抗生素治疗显示CFU计数优于T3。结论：墨西哥念珠菌提取物在体外和体内试验中均具有有益作用。

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