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首页> 外文期刊>Journal of Applied Life Sciences International >Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)
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Validation of a Simple Molecular Method for Assessment of Clonal Heterogeneity in Kiwifruit (Actinidia deliciosa A. Chev.)

机译：评估猕猴桃克隆异质性的简单分子方法的验证（Actinidia deliciosa A. Chev。）

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Aims: The industry of the green fleshed kiwifruit over the world is based on the cultivar Hayward due to outstanding characteristics of its fruits. Mutations and improper labeling of introduced plants could give rise to clonal heterogeneity within kiwifruit cultivars. Therefore, the objective is to validate a simple molecular approach to evaluate the clonal purity of cultivar Hayward in micropropagation laboratories and nurseries. Study Design: Molecular analysis of the genetic variability within cultivar. Place and Duration of Study: Biotechnology Laboratory at the Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata – Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Argentina, 2013. Methodology: Genomic DNA of four introductions and clone 8 of cultivar Hayward was amplified with randomly amplified polymorphic DNA markers. Cluster and principal coordinates analyses were performed. Results: The analyses revealed that all introductions were genetically different from each other. Conclusion: Small genetic differences within cultivars could be detected by this simple molecular approach when it is performed under well-controlled experimental conditions in each micropropagation laboratory or nursery.
机译：目标：由于果肉的杰出特性，全世界的绿色果肉猕猴桃产业以海沃德品种为基础。导入植物的突变和标记不当会导致猕猴桃品种的克隆异质性。因此，目的是验证一种简单的分子方法，以评估微繁殖实验室和苗圃中海沃德品种的克隆纯度。研究设计：品种内遗传变异的分子分析。研究的地点和持续时间：阿根廷马德普拉塔国立大学阿格拉里亚斯分校生物技术实验室–阿根廷农业技术研究所，阿根廷农业研究实验室，2013年。用随机扩增的多态性DNA标记进行扩增。进行聚类和主坐标分析。结果：分析表明，所有引种在基因上都互不相同。结论：在每个微繁实验室或苗圃中，在控制良好的实验条件下进行操作时，可以通过这种简单的分子方法检测出品种内部的微小遗传差异。

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