...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Life Sciences International >Biomass Saccharification and Screening for Production of Pectinase from Physocladia obscura JEL 513
【24h】

Biomass Saccharification and Screening for Production of Pectinase from Physocladia obscura JEL 513

机译：生物量糖化和黑胶果胶生产果胶酶的筛选JEL 513

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: This study documents for the first time the production of pectinase by Physocladia obscura JEL 513 and the optimization of its production in solid-state fermentation. Study Design: OFAT (One Factor At a Time). Place and Duration of Study: Botany Department, Faculty of Biological Science, University of British Columbia, Vancouver BC, Canada between January and June 2016. Methodology: Physocladia obscura JEL 513 was screened for its ability to grow on citrus peels and produce enzyme(s). After confirmation of pectinase production, several parameters of incubation time, pH and temperature on polygalacturonase activity were studied in screening and optimization investigations. Results: The maximum yield of pectinase was 760.785 U/g at 25oC in an orange waste peel medium of pH 6 after the fifth day of fermentation. High performance ion exchange liquid chromatography showed that, the concentration but not the relative monosaccharide composition changed in the investigated peels. Partial enzymatic degradation was evaluated by comparing the chemical thermal stability using a thermogravimetric technique of the fermented as well as the non-fermented citrus peels fibers. Conclusion: These results suggest that P. obscura JEL 513 shows promise in transforming agro-waste into a substrate for commercial enzyme production with potential broad benefits for regional economies.
机译：目的：本研究首次记录了暗生Physocladia obscura JEL 513产生的果胶酶及其在固态发酵中的生产优化。研究设计：OFAT（一次一个因素）。研究的地点和时间：2016年1月至2016年6月，加拿大不列颠哥伦比亚省温哥华市不列颠哥伦比亚大学生物科学学院植物学系。 ）。在确认果胶酶产生之后，在筛选和优化研究中研究了孵育时间，pH和温度对聚半乳糖醛酸酶活性的几个参数。结果：发酵第五天后，在pH为6的橙色废皮培养基中，在25oC下果胶酶的最大产量为760.785 U / g。高效离子交换液相色谱表明，所研究果皮中的浓度变化，但相对单糖组成没有变化。通过使用发酵的和未发酵的柑橘皮纤维的热重技术比较化学热稳定性来评估部分酶降解。结论：这些结果表明，P。obscura JEL 513在将农业废物转化为商业酶生产的底物方面显示出前景，对区域经济具有潜在的广泛利益。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号