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首页> 外文期刊>The Journal of automatic chemistry >A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement
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A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement

机译：高效液相色谱：化学发光法测定膳食补充剂中伐地那非的潜力

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摘要

A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H_(3)PO_(4) and ethylenediaminetetraacetic acid disodium salt (Na_(2)EDTA) aqueous solution (25?:?75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K_(3)Fe(CN)_(6) reaction in alkaline medium. At the flow rate of 0.8?mL/min, the vardenafil retention time ( t _( R )) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10~(?7) ~ 1.0 × 10~(?4)?mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.
机译：开发了一种高效液相色谱（HPLC）分离和化学发光（CL）检测流动性方法，用于膳​​食补充剂中敏感的伐地那非分析。使用乙醇-H_（3）PO_（4）和乙二胺四乙酸二钠盐（Na_（2）EDTA）水溶液（25？：？75，v / v％）在30°C的C18色谱柱上分离伐地那非作为流动相。随后的连续CL检测是基于在碱性介质中存在伐地那非对鲁米诺-K_（3）Fe（CN）_（6）反应的强烈CL增强而进行的。在流速为0.8？mL / min时，伐地那非保留时间（t_（R））为6.4分钟。研究和优化了影响HPLC分离度和CL检测的因素。伐地那非标准品的校准曲线在8.0×10〜（？7）〜1.0×10〜（？4）？mol / L的浓度范围内呈线性。日内和日间精度的相对标准偏差（RSD）小于3.5％。将该方法用于口服液体，葡萄酒和胶囊样品中伐地那非的测定。

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