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首页> 外文期刊>Journal of Behavioral and Brain Science >Sampling Small Volumes of Saliva for Determination of the Stress Hormone α-Amylase: A Comparative Methodological Study
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Sampling Small Volumes of Saliva for Determination of the Stress Hormone α-Amylase: A Comparative Methodological Study

机译：少量唾液采样以测定应激激素α-淀粉酶：比较方法学研究

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摘要

Two sampling devices that allow saliva collection through absorption to a cotton roll (Salivette?-method) or to small cotton pellets (VectaSpinTM Micro [VSM]-method) were studied. Any loss of salivary alpha-amylase (sAA) activity in relation to the saliva volume absorbed and harvested by centrifugation was examined. A pooled saliva sample prepared from stimulated whole saliva (collected by drooling) of 30 subjects was used. Three different saliva volumes (2.9 ml, 1.5 ml, and 0.8 ml) were tested on cotton rolls and two (0.03 ml, and 0.015 ml) on cotton pellets. The sample sAA activity was determined from the hydrolysis of 2-chloro-4-nitrophenyl-α-D-maltotrioside. In comparison with the original drooling sample, no sAA loss was observed in 1.5 ml samples tested with Salivette, while a significant decrease of activity was recorded with smaller volumes. VSM collected samples showed a non-volume dependent decrease of sAA activity of about 25%. Salivette requires large saliva volumes to allow an accurate sAA estimation. With cases of limited saliva access, VSM may be a suitable sampling device.
机译：研究了两种采样装置，它们可以通过吸收到棉卷（Salivette？方法）或小的棉丸（VectaSpinTM Micro [VSM]方法）中来收集唾液。检查了唾液α-淀粉酶（sAA）活性相对于通过离心吸收和收获的唾液量的任何损失。使用汇集的唾液样品，该样品由30名受试者的受刺激的整个唾液（通过流口水收集）制备而成。在棉卷上测试了三种不同的唾液体积（2.9 ml，1.5 ml和0.8 ml），在棉球上测试了两种（0.03 ml和0.015 ml）。由2-氯-4-硝基苯基-α-D-麦芽三糖苷的水解测定样品的sAA活性。与原始流口水样品相比，在用Salivette测试的1.5 ml样品中未观察到sAA的损失，而较小的样品量则表明活性显着下降。 VSM收集的样品显示sAA活性的非体积依赖性降低约为25％。 Salivette需要大量唾液才能进行准确的sAA估计。在唾液接触受限的情况下，VSM可能是合适的采样设备。

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