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首页> 外文期刊>The Journal of automatic chemistry >Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans
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Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

机译：微波辅助吉拉德衍生化与离子液体基质的结合用于人血清N-聚糖的灵敏MALDI-TOF MS分析

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摘要

We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS. The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000?m/z range. Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans. However, with the established method, i.e., DHB/An method, only 15 glycans were assigned. To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.
机译：我们开发了一种新的方法，用于MALDI-TOF MS检测源自人血清的N-聚糖。微波辅助的吉拉德T衍生化，固相萃取脱盐和离子液体基质（2，5-二羟基苯甲酸/苯胺）（GT-SPE-DHB / An）的协同组合比N-聚糖检测更为灵敏MALDI-TOF MS中的常规离子液体基质。我们指定的N-聚糖数量在900–2,000？m / z范围内证实了我们方法的优越灵敏度。使用我们的GT-SPE-DHB / An方法，我们能够成功分配31个聚糖。然而，采用已建立的方法，即DHB / An方法，仅分配了15个聚糖。据我们所知，这种GT-SPE-DHB / An方法是首次将N-聚糖的阳离子衍生化和离子液体基质结合起来用于MALDI-TOF MS中的N-聚糖分析。

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