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首页> 外文期刊>The Journal of automatic chemistry >Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD
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Comparison of Three Sample Preparation Procedures for the Quantification of L-Arginine, Asymmetric Dimethylarginine, and Symmetric Dimethylarginine in Human Plasma Using HPLC-FLD

机译：使用HPLC-FLD定量测定人血浆中L-精氨酸，不对称二甲基精氨酸和对称二甲基精氨酸的三种样品制备方法的比较

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摘要

Increased asymmetric dimethylarginine (ADMA) in human plasma has been associated with reduced generation of nitric oxide, leading to atherosclerotic diseases. ADMA may therefore be an important biomarker for cardiovascular disease. In the present study, three sample preparation techniques were compared regarding the quantification of L-arginine and ADMA in human plasma: (A) protein precipitation (PP) based on aqueous trichloroacetic acid (TCA), (B) PP using a mixture of ammonia and acetonitrile, and (C) solid-phase extraction (SPE). The samples were analysed by using high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The analytical performance of (A) was comparable with that of (C), demonstrating recoveries of >90%, coefficient of variations (CVs, %) of 0.994), precision (<5%), and sensitivity (lower limit of quantification (LLOQ)) of 0.14?μM and 12?nM for L-arginine and ADMA, respectively. Due to the simplicity and speed of procedure (A), this approach may serve as preferred sample preparation of human plasma samples before HPLC-FLD in providing important information regarding elevated ADMA concentrations.
机译：人血浆中不对称二甲基精氨酸（ADMA）的增加与一氧化氮的产生减少有关，从而导致动脉粥样硬化疾病。因此，ADMA可能是心血管疾病的重要生物标志物。在本研究中，比较了三种定量制备人血浆中L-精氨酸和ADMA的样品制备技术：（A）基于三氯乙酸水溶液（TCA）的蛋白质沉淀（PP），（B）使用氨水混合物的PP乙腈，以及（C）固相萃取（SPE）。使用具有荧光检测功能的高效液相色谱（HPLC-FLD）对样品进行分析。 （A）的分析性能与（C）相当，表明回收率> 90％，变异系数（CVs，％）为0.994），精密度（<5％）和灵敏度（定量下限（ L-精氨酸和ADMA的LLOQ））分别为0.14？μM和12？nM。由于步骤（A）的简便性和速度，该方法可作为HPLC-FLD之前人类血浆样品的优选样品制备，以提供有关升高的ADMA浓度的重要信息。

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