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首页> 外文期刊>Journal of atherosclerosis and thrombosis. >Impact of Polyunsaturated Fatty Acids on Oxidized Low Density Lipoprotein-Induced U937 Cell Apoptosis
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Impact of Polyunsaturated Fatty Acids on Oxidized Low Density Lipoprotein-Induced U937 Cell Apoptosis

机译：多不饱和脂肪酸对氧化型低密度脂蛋白诱导的U937细胞凋亡的影响

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Aim: Dietary supplements in polyunsaturated fatty acids (PUFA), particularly omega-3, are well known for their beneficial effects in preventing cardiovascular diseases (CVD). The aim of this study was to determine the role of PUFA on the modulation of apoptosis induced by hypochlorous acidoxidized LDL (HOCl-oxLDL) in U937 cells. Methods: We tested the effect of monocyte cell line U937 supplementation with eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), arachidonic acid (ARA) or oleic acid (OA) on the modulation of HOCl-oxLDL-induced apoptosis. Results: First, we showed the incorporation of fatty acids in the cellular membrane in U937 cells. Then, we showed that both EPA and ARA exerted a pro-apoptotic effect through the intrinsic mitochondrial apoptotic pathway including the dissipation of mitochondrial membrane potential followed by cardiolipin depletion, the downstream activation of caspase-3 and the increase in DNA fragmentation. The pro-apoptotic effect of EPA or ARA was completely blocked in U937/Bcl-2 cells. Conclusions: A new mechanism of dietary supplements in PUFA with likely consequences in apoptosis could be suggested through the mitochondrial pathway in monocytes.
机译：目的：多不饱和脂肪酸（PUFA）尤其是omega-3的膳食补充剂以预防心血管疾病（CVD）的有益作用而闻名。这项研究的目的是确定PUFA在次氯酸氧化LDL（HOCl-oxLDL）诱导的U937细胞凋亡中的作用。方法：我们测试了补充二十碳五烯酸（EPA），二十二碳六烯酸（DHA），花生四烯酸（ARA）或油酸（OA）的单核细胞系U937对HOCl-oxLDL诱导的细胞凋亡的调节作用。结果：首先，我们显示了U937细胞中细胞膜中脂肪酸的掺入。然后，我们表明EPA和ARA都通过内在的线粒体凋亡途径发挥了促凋亡作用，包括线粒体膜电位的耗散，随后的心磷脂耗竭，caspase-3的下游激活以及DNA片段化的增加。 EPA或ARA的促凋亡作用在U937 / Bcl-2细胞中被完全阻断。结论：可以通过单核细胞的线粒体途径提示PUFA膳食补充剂的新机制，该机制可能会导致细胞凋亡。

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