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首页> 外文期刊>Journal of Basic and Applied Sciences >Lifescience Global :: Abstract : Continuous Ethanol Fermentation in Immersed, Cross-Flow Microfiltration Membrane Bioreactor with Cell Retention
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Lifescience Global :: Abstract : Continuous Ethanol Fermentation in Immersed, Cross-Flow Microfiltration Membrane Bioreactor with Cell Retention

机译：Lifescience Global ::摘要：沉浸式，错流微滤膜生物反应器中的乙醇连续发酵，具有细胞保留功能

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The key objective of this study was to devise a continuous ZeeWeed?? membrane-based, immersed, microfiltration (MF) laboratory scale fermentation system for ethanol production with cell retention to achieve effective ethanol productivity, flux rates and sugar utilization. The new bioreactor was compared to the fermentation kineticsa?? of the ultrafiltration unit.A synthetic glucose based medium was fermented by fresh, bakera??s yeast to produce ethanol. The cells were not recycled; the medium was continuously withdrawn by filtration through an internal, immersed hollow-fiber cartridge. In this way, the inside of the membrane was exposed to the ethanolic solution, while broth with viable yeast cells remained outside the membrane. This design, with a cell retention system, provided much less membrane fouling (loss of about 76% of the original water flux after 96 hours of filtration) than while using the ultrafiltration (UF) external hollow-fiber membrane with cell recycling (loss of 97% of the original water flux after 2-3 hours of operation). Both modules converted at least 95% of glucose with biomass concentration of 30 g/L, and the final ethanol concentration of 62 g/L. However, the UF membrane became plugged after only 2 hrs of operation. The ZeeWeed?? membrane operated successfully for 96 hrs with a final flux of 4 L/h m2 with ethanol concentration of 62.4 g/l, biomass yield 0.34 g/g and cell viability of 95.3%. This concept could be successfully used for biofuel production. A very strong positive correlation was observed between the biomass and EtOH concentration (R=0.98; at p0.05).
机译：这项研究的主要目的是设计一个连续的ZeeWeed？基于膜的，浸入式微滤（MF）实验室规模的发酵系统，用于乙醇生产并保留细胞，以实现有效的乙醇生产率，通量率和糖利用率。将新的生物反应器与发酵动力学进行了比较。将基于葡萄糖的合成培养基用新鲜的面包酵母发酵以产生乙醇。电池没有回收;通过内部浸入式中空纤维滤芯过滤，连续抽出培养基。这样，膜的内部暴露于乙醇溶液，而带有活酵母细胞的肉汤保留在膜的外部。与采用超滤（UF）外部中空纤维膜并进行细胞回收处理（损失90％）相比，这种具有细胞保留系统的设计所产生的膜污染（过滤96小时后损失的水流量约为原始水通量的76％）要少得多。运行2-3小时后，原始水通量的97％）。两个模块均转化了至少95％的葡萄糖，其中生物质浓度为30 g / L，最终乙醇浓度为62 g / L。但是，仅运行2小时后，UF膜便被堵塞。 ZeeWeed ？？膜成功运行96小时，最终通量为4 L / h m2，乙醇浓度为62.4 g / l，生物质产量为0.34 g / g，细胞活力为95.3％。该概念可以成功地用于生物燃料生产。在生物质和EtOH浓度之间观察到非常强的正相关性（R = 0.98;在p <0.05时）。

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