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首页> 外文期刊>Journal of Biotechnology and Biodiversity >Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens
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Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens

机译：桉树小枝茎尖遗传转化的初步结果。通过根癌农杆菌

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The regeneration of transgenic plants of Eucalypt is the largest difficulty for the genetic transformation of this genus; in addition a low rate of transformed plants is reached. The aim of this research was to evaluate acetosyringone (3',5'-Dimethoxy-4'-hydroxyacetophenone) on the co-culture medium during genetic transformation of shoot tips of Eucalyptus saligna via Agrobacterium tumefaciens and to promote the explants selection supposedly transformed. Shoot tip from multiple shoots was used as explants. These explants were pre-cultured during two days before the transformation. Strain EHA105 of A. tumefaciens harboring the plasmid pBI121 was used. The treatments were: 0 and 100 μM acetosyringone added to the co-culture, after co-culture the explants were cultured in multiplication medium supplemented with 250 mg.L -1 Cefotaxime ? and each sub-culture the kanamycin levels were increased from 50 to 150 mg.L -1 . The transient expression of the uidA gene in shoot tips was evaluated after the end of the co-culture (fifth day) and after seven days of culture on medium containing kanamycin. The presence of 100 μM acetosyringone at the co-culture of shoot tips of Eucalyptus saligna promoted higher transient expression of the uidA gene and retards toxic effects caused by kanamycin.
机译：桉树转基因植物的再生是该属遗传转化的最大困难。另外，转化植物的比率低。这项研究的目的是在通过根癌农杆菌对桉树的芽尖进行遗传转化期间，在共培养基上评估乙酰丁香酮（3'，5'-二甲氧基-4'-羟基苯乙酮），并促进据认为已转化的外植体选择。来自多个芽的芽尖用作外植体。这些外植体在转化前两天进行了预培养。使用带有质粒pBI121的根癌农杆菌EHA105菌株。处理为：向共培养物中加入0和100μM乙酰丁香酮，共培养后，将外植体在补充有250 mg.L -1头孢噻肟的增殖培养基中培养。每次传代培养时，卡那霉素水平从50 mg.L -1增加到150 mg.L -1。共培养结束后（第五天）和在含卡那霉素的培养基上培养7天后，评估茎尖中uidA基因的瞬时表达。桉树茎尖共培养物中存在100μM乙酰丁香酮，可促进uidA基因的更高瞬时表达并延缓卡那霉素引起的毒性作用。

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