• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Botany >Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem
【24h】

Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem

机译：改进和可复制的流式细胞术从单根分生组织中分离核的方法学。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Root meristems have increasingly been target of cell cycle studies by flow cytometric DNA content quantification. Moreover, roots can be an alternative source of nuclear suspension when leaves become unfeasible and for chromosome analysis and sorting. In the present paper, a protocol for intact nuclei isolation from a single root meristem was developed. This proceeding was based on excision of the meristematic region using a prototypical slide, followed by short enzymatic digestion and mechanical isolation of nuclei during homogenization with a hand mixer. Such parameters were optimized for reaching better results. Satisfactory nuclei amounts were extracted and analyzed by flow cytometry, producing histograms with reduced background noise and CVs between 3.2 and 4.1%. This improved and reproducible technique was shown to be rapid, inexpensive, and simple for nuclear extraction from a single root tip, and can be adapted for other plants and purposes.
机译：通过流式细胞术DNA含量定量，根分生组织越来越成为细胞周期研究的目标。此外，当叶片变得不可行时，根可以用作核悬浮的替代来源，并可以用于染色体分析和分选。在本文中，开发了从单个根分生组织完整核分离的协议。此过程基于使用原型载玻片切除分生组织区域，然后在用手动混合器匀浆期间进行短暂的酶消化和核的机械分离。优化这些参数以获得更好的结果。提取令人满意的细胞核并通过流式细胞仪进行分析，产生直方图，背景噪声降低，CV降低3.2％至4.1％。该改进和可再现的技术显示出快速，廉价且简单的从单个根尖提取核的技术，并且可适用于其他植物和目的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号