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首页> 外文期刊>Journal of Botany >Phenolic Composition and Antioxidant and Antimicrobial Activities of Extracts Obtained fromCrataegus azarolusL. var.aronia(Willd.) Batt. Ovaries Calli
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Phenolic Composition and Antioxidant and Antimicrobial Activities of Extracts Obtained fromCrataegus azarolusL. var.aronia(Willd.) Batt. Ovaries Calli

机译：从Crataegus azarolusL获得的提取物的酚类成分，抗氧化和抗菌活性。 var.aronia（Willd。）卵巢Calli

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Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions.Methods and Results. The production of polyphenol by fifty-two-week-oldCrataegus azarolusvar.aroniacalli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEACDPPH, TEACABTS, and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (−)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period.Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids.Crataegus azarolusvar.aroniacells culture represents an important alternative source of natural antioxidants and antimicrobials.
机译：目的。植物细胞培养是产生多种物质的创新技术。许多植物在其次生代谢产物中合成具有抗氧化和抗菌作用的酚类化合物。山楂（Crataegus）是这些植物中的一种，长期用于民间医学，并广泛用于药物制剂中，主要用于神经和心脏的镇静作用。方法与结果。研究了52周龄的Crataegus azarolusvar.aroniacalli多酚的产生与继代培养期内生长变化以及抗氧化和抗菌能力的关系。 DPPH和ABTS +分析用于表征愈伤组织培养物的抗氧化作用。通过使用圆盘扩散和稀释测定法测试抗菌活性，以确定每种活性提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值。当达到最大生长时，观察到高的TEACDPPH，TEACABTS和抗菌活性。在此期间产生了最佳的总酚，原花青素，类黄酮，（-）-表儿茶素，原花青素B2，绿原酸和高丝苷。抗氧化和抗微生物活性与总酚和总黄酮密切相关。Crataegus azarolusvar.aroniacells细胞培养物是天然抗氧化剂和抗微生物剂的重要替代来源。

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