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首页> 外文期刊>Journal of Cancer Research and Therapeutics >Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids
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Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids

机译：龙眼花原花青素诱导HT-29结直肠癌球体凋亡

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Aim of Study: Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model. Materials and Methods: A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot. Results: Treatment with LFP up to 200 μg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2. Conclusion: The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components.
机译：研究目的：富含原花青素的龙眼花提取物（LFP）先前已显示出在体外抑制两种大肠癌（CRC）细胞在软琼脂中的增殖和锚定非依赖性生长。在本报告中，我们进一步研究了LFP在CRC椭球模型中的作用。材料和方法：采用HT-29椭球的液体覆盖测定法评估LFP对癌细胞肿瘤发生，生存能力和凋亡的影响。使用蛋白质印迹法测量对信号通路（表皮生长因子受体[EGFR]，Akt）和凋亡调节剂的相关影响。结果：通过膜联蛋白V染色测量，高达200μg/ ml的LFP处理以剂量依赖性方式抑制肿瘤生长，并诱导显着的细胞凋亡。 LFP处理的细胞显示EGFR和Akt磷酸化降低，B细胞淋巴瘤2表达降低。结论：LFP诱导CRC球体凋亡的能力值得进一步研究其组成和鉴定肿瘤活性成分。

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