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首页> 外文期刊>Journal of Cancer Research and Therapeutics >Candidate gene biodosimeters of mice and human exposure to ionizing radiation by quantitative reverse transcription polymerase chain reaction
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Candidate gene biodosimeters of mice and human exposure to ionizing radiation by quantitative reverse transcription polymerase chain reaction

机译：通过定量逆转录聚合酶链反应，小鼠和人类暴露于电离辐射中的候选基因生物剂量计

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Understanding of cellular responses to ionizing radiation (IR) is essential for the development of predictive markers useful for assessing human exposure. Biological markers of exposure to IR in human populations are of great interest for assessing normal tissue injury in radiation oncology and for biodosimetry in nuclear incidents and accidental radiation exposures. Traditional radiation exposure biomarkers based on cytogenetic assays (biodosimetry), are time-consuming and do not provide results fast enough and requires highly trained personnel for scoring. Hence, the development of rapid biodosimetry methods is one of the highest priorities. Exposure of cells to IR activates multiple signal transduction pathways, which result in complex alterations in gene-expression. Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in monitoring the specific genes with more accurately and sensitively. This review evaluates the RT-qPCR as a biodosimetry method and we investigated the papers from 2000 up to now, which identified the genes-expression related the DNA repair, cell cycle checkpoint, and apoptosis induced by ionization radiation in peripheral blood and determined as biodosimeters. In conclusion, it could be say that RT-qPCR technique for determining the specific genes as biodosimeters could be a fully quantitative reliable and sensitive method. Furthermore, the results of the current review will help the researchers to recognize the most expressed genes induced by ionization radiation.
机译：了解细胞对电离辐射（IR）的反应对于开发可用于评估人体暴露的预测标记至关重要。人群中暴露于IR的生物标志物对于评估放射肿瘤学中的正常组织损伤以及核事件和意外辐射暴露中的生物剂量测定具有重大意义。基于细胞遗传学测定法（生物剂量测定法）的传统辐射暴露生物标志物既费时又不能提供足够快的结果，并且需要训练有素的人员进行评分。因此，快速生物剂量测定方法的发展是最高优先事项之一。细胞暴露于IR会激活多种信号转导途径，从而导致基因表达的复杂变化。实时定量逆转录聚合酶链反应（RT-qPCR）已成为检测和定量RNA靶标的基准，并且越来越多地被用于更准确，更灵敏地监测特定基因。这篇综述评估了RT-qPCR作为一种生物剂量测定方法，我们对2000年至今的论文进行了研究，鉴定了与DNA修复，细胞周期检查点和外周血电离辐射诱导的细胞凋亡相关的基因表达，并确定为生物剂量计。总之，可以说用RT-qPCR技术确定作为生物剂量计的特定基因可能是一种完全定量的可靠而灵敏的方法。此外，当前审查的结果将有助于研究人员识别由电离辐射诱导的表达最多的基因。

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