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首页> 外文期刊>Journal of Cancer Research and Treatment >Doublecortin like Kinase-1 is Overexpressed in Breast Cancer Tissues and Correlated with Epithelial-mesenchymal Transition Markers
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Doublecortin like Kinase-1 is Overexpressed in Breast Cancer Tissues and Correlated with Epithelial-mesenchymal Transition Markers

机译：Doublecortin样激酶1在乳腺癌组织中过表达，并与上皮-间质转化标记物相关

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Background: Much of the current literature support, the idea that epithelial to mesenchymal transition (EMT) is the key mechanism by which tumor cells gain invasive and metastatic ability, as EMT enables separation of individual cells from the primary tumor mass as well as promote migration. After undergoing EMT, thereby enabling access to hematogenous or lymphatic routes of dissemination, tumor cells can extravasate into secondary organs and establish micro-metastases. Objective: The main target of this work was to study the relation between expression of Doublecortin-like kinase-1 (DCLK-1) and epithelial to mesenchymal transition markers (E-cadherin, vimentin and transforming growth factor-beta (TGF-β)) in breast cancer patients and assess its role in cancer prognosis. Materials and Methods: This study included 60 breast cancer patients and 40 healthy females as control group. Tumor tissues and adjacent normal breast tissues were collected from patients with breast cancer. A single venous blood sample was collected concurrently from breast cancer patients (Before surgery) and from the control group. Tissue expression of DCLK-1, E-cadherin and vimentin were evaluated in breast cancer tissues and normal breast tissues by real-time reverse transcription polymerase chain reaction (RT-PCR). Serum level of TGF-β was assayed by enzyme linked-immunossorbant assay. Results: According to the results of the present study DCLK-1 is highly expressed in breast cancer tissues compared to normal breast tissues. Overexpression of DCLK-1 was significantly correlated with higher tissues Vimentin expression, increased serum TGF-β and lower tissues E-cadherin expression. Kaplan-Meier survival curves for breast cancer patients revealed that, patients with elevated tissue DCLK-1 and Vimentin expression and higher serum TGF-β were significantly associated with poor prognosis in primary breast cancer patients. However, patients with lower tissue E-Cadherin expression had shorter disease free survival time than patients with higher levels. Conclusion: DCLK-1 was significantly increased in breast cancer tissues in comparison to normal breast tissues and its overexpression was significantly correlated with EMT markers and poor prognosis in breast cancer patients. Further prospective studies using greater numbers of patients are required to confirm our findings.
机译：背景：目前的许多文献支持这种观点，即上皮向间质转化（EMT）是肿瘤细胞获得侵袭和转移能力的关键机制，因为EMT可以使单个细胞与原发肿瘤块分离并促进迁移。接受EMT从而进入血源性或淋巴性传播途径后，肿瘤细胞可渗入次生器官并建立微转移。目的：这项工作的主要目的是研究Doublecortin样激酶1（DCLK-1）的表达与上皮到间充质转换标记物（E-cadherin，波形蛋白和转化生长因子-β（TGF-β）之间的关系。 ），并评估其在癌症预后中的作用。材料与方法：本研究纳入60例乳腺癌患者和40例健康女性作为对照组。从乳腺癌患者中收集肿瘤组织和邻近的正常乳腺组织。同时从乳腺癌患者（手术前）和对照组中采集单个静脉血样本。通过实时逆转录聚合酶链反应（RT-PCR）评估乳腺癌组织和正常乳腺组织中DCLK-1，E-钙黏着蛋白和波形蛋白的组织表达。通过酶联免疫吸附测定法测定血清TGF-β水平。结果：根据本研究的结果，与正常乳腺组织相比，DCLK-1在乳腺癌组织中高表达。 DCLK-1的过表达与较高的组织波形蛋白表达，较高的血清TGF-β和较低的组织钙粘蛋白表达密切相关。乳腺癌患者的Kaplan-Meier生存曲线显示，组织DCLK-1和波形蛋白表达升高以及血清TGF-β升高的患者与原发性乳腺癌患者的不良预后显着相关。然而，与较高水平的患者相比，组织E-钙黏着蛋白表达较低的患者的无病生存时间较短。结论：与正常乳腺组织相比，DCLK-1在乳腺癌组织中显着增加，并且其过表达与乳腺癌患者的EMT标志物和不良预后显着相关。需要使用更多患者的进一步前瞻性研究来证实我们的发现。

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