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首页> 外文期刊>Journal of Cancer Research and Therapeutics >Baicalein and Ly294002 induces liver cancer cells apoptosis via regulating phosphatidyl inositol 3-kinase/Akt signaling pathway
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Baicalein and Ly294002 induces liver cancer cells apoptosis via regulating phosphatidyl inositol 3-kinase/Akt signaling pathway

机译：黄ical素和Ly294002通过调节磷脂酰肌醇3-激酶/ Akt信号通路诱导肝癌细胞凋亡

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Aim: The aim of this study is to investigate the mechanism of baicalein in inducing human liver cell line SMMC-7721 apoptosis. Materials and Methods: Twenty micromoles baicalein or 10 μM LY294002 was adopted to treat SMMC-7721 cells. Cell proliferation was tested by cell counting kit-8 assay. Cell cycle was determined by flow cytometry and cyclin D1 expression. Cell apoptosis was detected by annexin V/propidium iodide double staining. Phosphatidyl inositol 3-kinase (PI3K)/Akt signaling pathway was assessed by real-time polymerase chain reaction and Western blot. Results: Baicalein suppressed liver cancer cell SMMC-7721 proliferation and induced cell apoptosis together with LY294002. Baicalein blocked cell cycle in G0–G1 phase and downregulated cyclin D1 level. Baicalein and LY294002 significantly suppressed PI3K/Akt signaling pathway-related molecule activity at both mRNA and protein levels (P Conclusion: Baicalein can inhibit liver cancer cell proliferation and promote cell apoptosis by affecting PI3K/Akt signaling pathway together with LY294002.
机译：目的：本研究的目的是研究黄in素诱导人肝细胞SMMC-7721凋亡的机制。材料与方法：采用二十微摩尔黄ical素或10μMLY294002处理SMMC-7721细胞。通过细胞计数试剂盒8测定法测试细胞增殖。通过流式细胞术和细胞周期蛋白D1表达确定细胞周期。通过膜联蛋白V /碘化丙啶双染色检测细胞凋亡。通过实时聚合酶链反应和蛋白质印迹评估了磷脂酰肌醇3-激酶（PI3K）/ Akt信号通路。结果：黄ical素与LY294002一起抑制肝癌细胞SMMC-7721增殖并诱导细胞凋亡。黄ical素在G0–G1期阻止了细胞周期并下调了cyclin D1水平。黄ical素和LY294002在mRNA和蛋白质水平上均显着抑制PI3K / Akt信号通路相关分子的活性（P结论：黄ical素可通过与LY294002一起影响PI3K / Akt信号通路来抑制肝癌细胞增殖并促进细胞凋亡。

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