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首页> 外文期刊>Journal of Clinical Medicine >Thyroid Hormones and Spermatozoa: In Vitro Effects on Sperm Mitochondria, Viability and DNA Integrity
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Thyroid Hormones and Spermatozoa: In Vitro Effects on Sperm Mitochondria, Viability and DNA Integrity

机译：甲状腺激素和精子：对精子线粒体，生存力和DNA完整性的体外影响。

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The aim of this study wasto assess the in vitro effects of levothyroxine (LT4) on conventional and bio-functional sperm parameters and its implications on fertility. Patients with male idiopathic infertility were enrolled and subjected to examination of the seminal fluid and capacitation according to the WHO 2010 criteria and flow cytometric sperm analysis for the evaluation of bio-functional sperm parameters. LT4 significantly increased the percentage of spermatozoa with high mitochondrial membrane potential (MMP), decreased the percentage of spermatozoa with low MMP and increased sperm motility already at a concentration of 0.9 pmol L ?1 . Therefore, LT4 significantly reduced sperm necrosis and lipid peroxidation ameliorating chromatin compactness. These effects of LT4 were evident at a concentration of 2.9 pmol L ?1 , close to the physiological free-thyroxine (FT4) concentrations in the seminal fluid of euthyroid subjects. We showed a beneficial role of thyroid hormones on sperm mitochondrial function, oxidative stress and DNA integrity. The results of this in vitro study could have a clinical application in patients with idiopathic infertility, clarifying the role of thyroid function on male fertility.
机译：本研究的目的是评估左甲状腺素（LT4）对常规和生物功能性精子参数的体外影响及其对生育力的影响。纳入男性特发性不育患者，并根据WHO 2010标准对精液和获能进行检查，并进行流式细胞术精子分析，以评估生物功能性精子参数。在浓度为0.9 pmol L？1的情况下，LT4显着提高了具有高线粒体膜电位（MMP）的精子的百分比，降低了具有低MMP的精子的百分比并提高了精子活力。因此，LT4显着减少了精子坏死和脂质过氧化，改善了染色质的致密性。 LT4的这些作用在2.9 pmol L？1的浓度下很明显，接近于正常甲状腺受试者精液中的生理性游离甲状腺素（FT4）浓度。我们显示了甲状腺激素对精子线粒体功能，氧化应激和DNA完整性的有益作用。这项体外研究的结果在特发性不育患者中可能具有临床应用，阐明了甲状腺功能对男性生育的作用。

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