• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Medicine and Research >Decrease in expression of some proteins among breast cancer patients in Sudan comparison to healthy tissues
【24h】

Decrease in expression of some proteins among breast cancer patients in Sudan comparison to healthy tissues

机译：苏丹乳腺癌患者某些蛋白质的表达与健康组织相比下降

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Breast cancer is one of the most common cancers in women, and rated the second most common cancer and a significant cause of death in females in the Sudan. This study aims to identify tumor protein that elicits humoral immune responses in breast cancer patient in comparison to tissues from healthy individuals as well as from normal tissues of the cancer patient. Serum samples and breast cancer tissue specimens were collected from breast cancer patients (n = 9) and from healthy individuals (n = 5) at Khartoum Teaching Hospital. Breast cancer tissues were homogenized in PBS, centrifuged and the supernatants were lysed in 2X SDS-PAGE sample buffer. The preparation then boiled and the resulting supernatants were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Total proteins were separated on SDS-PAGE and transferred to the nitrocellulose paper, then analyzed by immunoblotting for total proteins and serum antibodies using serum from patients with breast cancer and from healthy individuals to enhance humoral immune responses. The SDS-PAGE analysis showed an increase in size of protein bands in the normal control tissues than from breast cancer patients. The Western blot analysis of breast cancer tissues with the serum from breast cancer patients specifically detected major bands than in the normal tissues from the healthy tissues of cancer patients or from healthy individuals. Beside the major bands, additional bands have been detected in breast cancer tissues with the serum from breast cancer patients. The reactive auto-antibodies in patient’s tissues bound to the circulating tumor antigen in patient’s serum and immune complexes would result by Western blotting indication of strong immune response to these proteins. The present study demonstrated that there was a clear decline in the expression of some proteins among breast cancer patients which has been confirmed by strong immune reactions in the Western blotting analysis.
机译：乳腺癌是女性中最常见的癌症之一，在苏丹女性中，乳腺癌是第二大常见癌症，也是重要的死亡原因。这项研究的目的是鉴定与健康人以及癌症患者正常组织相比，在乳腺癌患者中引起体液免疫反应的肿瘤蛋白。从喀土穆教学医院的乳腺癌患者（n = 9）和健康个体（n = 5）收集血清样本和乳腺癌组织标本。将乳腺癌组织在PBS中匀浆，离心，然后在2X SDS-PAGE样品缓冲液中裂解上清液。然后将制剂煮沸，并将所得的上清液进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹分析。在SDS-PAGE上分离总蛋白并转移到硝酸纤维素纸上，然后通过免疫印迹分析乳腺癌和健康个体血清中的总蛋白和血清抗体，以增强体液免疫反应。 SDS-PAGE分析显示正常对照组织中蛋白质条带的大小比乳腺癌患者的蛋白质条带的大小增加。用来自乳腺癌患者的血清对乳腺癌组织进行的蛋白质印迹分析特异性地检测到了比来自癌症患者的健康组织或健康个体的正常组织中的主要条带。除主要谱带外，在乳腺癌组织中还检测到其他谱带，这些谱带来自乳腺癌患者的血清。 Western印迹表明，对这些蛋白质具有强烈免疫反应的结果可能是与患者血清和免疫复合物中循环肿瘤抗原结合的患者组织中的反应性自身抗体。本研究表明，在乳腺癌患者中某些蛋白质的表达明显下降，这已通过蛋白质印迹分析中的强免疫反应得以证实。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号