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首页> 外文期刊>Journal of Clinical and Experimental Investigations >Human embryonic stem cells and microenvironment
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Human embryonic stem cells and microenvironment

机译：人胚胎干细胞与微环境

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Human embryonic stem cells (hESCs) possess a great potential in the field of regenerative medicine by their virtue of pluripotent potential with indefinite proliferation capabilities. They can self renew themselves and differentiate into three embryonic germ layers. Although they are conventionally grown on mitotically inactivated mouse feeder cells, there are in vitro culture systems utilizing feeder cells of human origin in order to prevent cross-species contamination. Recently established in vitro culture systems suggested that direct interaction with feeder cells is not necessary but rather attachment to a substrate is required to ensure long-term, efficient hESC culture in vitro. This substrate is usually composed of a mixture of extracellular matrix components representing in vivo natural niche. In hESC biology, the mechanism of interaction of hESCs with extracellular matrix molecules remained insufficiently explored area of research due to their transient nature of interaction with the in vivo niche. However, an in vitro culture system established using extracellular matrix molecules may provide a safer alternative to culture systems with feeder cells while paving the way to Good Manufacturing Practice-GMP production of hESCs for therapeutic purposes. Therefore, it is essential to study the interaction of extracellular matrix molecules with hESCs in order to standardize in vitro culture systems for large-scale production of hESCs in a less labor-intensive way. This would not only provide valuable information regarding the mechanisms that control pluripotency but also serve to dissect the molecular signaling pathways of directed differentiation for prospective therapeutic applications in the future. J Clin Exp Invest 2014; 5 (3): 486-495
机译：人类胚胎干细胞（hESCs）具有多能潜能，具有无限的增殖能力，在再生医学领域具有巨大潜力。它们可以自我更新并分化为三个胚芽层。尽管它们通常在有丝分裂失活的小鼠饲养细胞上生长，但是为了防止跨物种污染，存在一些利用人源饲养细胞的体外培养系统。最近建立的体外培养系统表明，与饲养细胞的直接相互作用不是必需的，而是需要附着在底物上以确保长期有效的体外hESC培养。该底物通常由代表体内天然生态位的细胞外基质成分的混合物组成。在hESC生物学中，hESC与细胞外基质分子相互作用的机制由于其与体内生态位相互作用的瞬时性质而仍未得到充分研究。但是，使用细胞外基质分子建立的体外培养系统可以为带饲养细胞的培养系统提供更安全的替代方法，同时为治疗目的向hESC的良好生产规范-GMP生产铺平了道路。因此，有必要研究细胞外基质分子与hESC的相互作用，以标准化以较少劳动强度的方式大规模生产hESC的体外培养系统。这不仅将提供有关控制多能性的机制的有价值的信息，而且还将有助于剖析定向分化的分子信号传导途径，以供未来的治疗用途。 2014年《临床投资杂志》; 5（3）：486-495

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