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首页> 外文期刊>Journal of clinical laboratory analysis. >Mathematical Equations to Calculate True Mycophenolic Acid Concentration in Human Plasma by Using Two Immunoassays With Different Cross-Reactivities With Acyl Glucuronide Metabolite: Comparison of Calculated Values With Values Obtained by Using an HPLC-UV Method
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Mathematical Equations to Calculate True Mycophenolic Acid Concentration in Human Plasma by Using Two Immunoassays With Different Cross-Reactivities With Acyl Glucuronide Metabolite: Comparison of Calculated Values With Values Obtained by Using an HPLC-UV Method

机译：通过使用两种具有交叉交叉反应性的酰基葡糖醛酸代谢产物的不同免疫测定法来计算人血浆中真实麦考酚酸浓度的数学方程式：计算值与通过HPLC-UV方法获得的值的比较

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Both immunoassays and chromatographic methods are available for therapeutic drug monitoring of mycophenolic acid (MPA). Although chromatographic methods are more precise, immunoassays are widely used in clinical laboratories due to ease of adopting such assays on automated analyzers. We studied the possibility of using mathematical equations to calculate true MPA concentration by accounting for acyl glucuronide cross-reactivities with immunoassays by using two immunoassays with widely different cross-reactivities with the metabolite.MethodsWe determined MPA concentrations in 20 specimens obtained from transplant recipients using cloned enzyme donor immunoassay (CEDIA) assay and a new particle enhanced turbidimetric inhibition immunoassay (PETINIA) assay. Then we developed mathematical equations to calculate true MPA concentration using values obtained by both immunoassays and reported cross-reactivity of acyl glucuronide with respective immunoassays. Calculated concentrations were compared with values obtained by using a high-performance liquid chromatography combined with ultraviolet detection (HPLC-UV) method.ResultsWe obtained good correlation between calculated MPA concentrations and corresponding MPA level obtained by using HPLC-UV method. Using x-axis as the MPA concentrations determined by the HPLC-UV method and y-axis as the calculated MPA level, we observed the following regression equation: y = 1.083x ? 0.0995 (r = 0.99, n = 20).ConclusionsMathematical equations can be used to calculate true MPA concentrations using two immunoassays with different cross-reactivities with acyl glucuronide metabolite.
机译：免疫测定和色谱方法均可用于霉菌酚酸（MPA）的治疗药物监测。尽管色谱方法更为精确，但由于易于在自动分析仪上采用此类测定，免疫测定已广泛用于临床实验室。我们研究了使用数学方程式来计算真实的MPA浓度的可能性，方法是使用两种代谢物的交叉反应性不同的免疫测定方法，通过免疫测定法解决酰基葡萄糖醛酸化物的交叉反应性方法。方法我们使用克隆的方法确定了从移植受体获得的20个样本中的MPA浓度酶供体免疫分析（CEDIA）分析和新型颗粒增强浊度抑制免疫分析（PETINIA）分析。然后，我们开发了数学方程式，以使用通过两种免疫测定获得的值来计算真实的MPA浓度，并报告了酰基葡糖醛酸苷与相应的免疫测定的交叉反应性。将计算出的浓度与通过高效液相色谱结合紫外检测（HPLC-UV）方法获得的值进行比较。结果我们获得了计算出的MPA浓度与通过HPLC-UV方法获得的相应MPA水平之间的良好相关性。用x轴作为通过HPLC-UV法测定的MPA浓度，将y轴作为计算出的MPA水平，我们观察到以下回归方程：y = 1.083x？ 0.0995（r = 0.99，n = 20）。结论可以使用两种具有酰基葡萄糖醛酸苷代谢物交叉反应性不同的免疫测定法，使用数学方程式计算真实MPA浓度。

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