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首页> 外文期刊>Journal of Dentistry >Study of Extrinsic Apoptotic Pathway in Oral Pemphigus Vulgaris Using TNFR 1 and FasL Immunohistochemical Markers and TUNEL Technique
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Study of Extrinsic Apoptotic Pathway in Oral Pemphigus Vulgaris Using TNFR 1 and FasL Immunohistochemical Markers and TUNEL Technique

机译：TNFR 1和FasL免疫组化标记及TUNEL技术研究寻常型口服天疱疮的外源凋亡途径。

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Statement of the Problem: Pemphigus vulgaris is characterized by intraepithelial vesicles, but pathogenesis of vesicle formation in this disease has not been substantiated yet.Purpose: The present study investigate extrinsic apoptotic pathway in oral pemphigus vulgaris using TUNEL and important immunohistochemical markers of extrinsic pathway, TNFR1 and FasL.Materials and Method: In the present cross sectional study, 25 oral pemphigus vulgaris samples and 6 normal oral mucosa were analyzed for the presence of apoptosis by TUNEL and the staining of TNFR1 and FasL in basal and parabasal layers around vesicle, vesicle floor, vesicle roof and acantholytic cells. The staining expression and intensity were measured and the obtained data were analyzed by Wilcoxon and Mann-Whitney tests.Results: There was no or faint staining of TUNEL, FasL and TNFR1 in normal oral mucosa. In addition, there was no significant difference between the staining of TUNEL technique in different layers. The staining of TNFR1 marker was very high in all regions. FasL marker was not positive in the basal and parabasal layers around vesicle in 92% of samples but showed a varied and different staining in vesicle region. There was a significant difference between the each two markers in all layers ( p 0.001).Conclusion: Apoptosis is probably is a preceding phenomenon to acantholysis in pemphigus vulgaris. It appears that the apoptosis occurs mostly by extrinsic pathway using proapototic mediators TNFR1 and FasL.??
机译：问题陈述：寻常型天疱疮以上皮内囊泡为特征，但该病中囊泡形成的发病机理尚未得到证实。目的：本研究使用TUNEL和重要的外在途径免疫组织化学标记物研究寻常性天疱疮的外在凋亡途径， TNFR1和FasL。材料与方法：在本横断面研究中，通过TUNEL分析了25例寻常性天疱疮样本和6例正常口腔粘膜是否存在凋亡，以及囊泡，囊泡周围基底层和副基底层中TNFR1和FasL的染色情况地板，囊顶和棘皮细胞。结果：正常口腔黏膜中TUNEL，FasL和TNFR1均未见或微弱染色，检测其染色表达和强度，并通过Wilcoxon和Mann-Whitney检测对所得数据进行分析。另外，TUNEL技术在不同层的染色之间没有显着差异。在所有区域中，TNFR1标记的染色都很高。在92％的样品中，FasL标记在囊泡周围的基底层和副基底层中不是阳性，但在囊泡区域显示出不同的染色。结论：细胞凋亡可能是寻常型天疱疮棘层松解术的先兆现象（p <0.001）。似乎凋亡是通过使用促凋亡的介质TNFR1和FasL通过外在途径发生的。

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