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首页> 外文期刊>Journal of Applied Medical Sciences >In Vivo Spectroscopic Study on the Effects of Ionising and Non-Ionising Radiation on Some Biophysical Properties of Rat Blood
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In Vivo Spectroscopic Study on the Effects of Ionising and Non-Ionising Radiation on Some Biophysical Properties of Rat Blood

机译:电离和非电离辐射对大鼠血液某些生物物理特性影响的体内光谱研究

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The present study aimed to analyse the radiation risk associated with the exposure of haemoglobin (Hb) of rat red blood cells (rbcs) exposed to a 50-Hz 6-kV/m electric field, a fast neutron dose of 1 mSv, and mixed radiation from fast neutrons and an electric field distributed over a period of three weeks at a rate of 5 days/week and 8 hours/day. The dielectric measurements and the absorption spectra for the haemoglobin molecule in the frequency range of 1 kHz to 5 MHz were measured for all of the samples. The dielectric relaxation results demonstrated an increase in the dielectric increment (Δε) for the rbcs from all of the irradiated animals, which indicates an increase in the electric dipole. Moreover, the results revealed a decrease in the relaxation time (τ) and the molecular radius (r) of the irradiated molecules, which indicates that the increase in Δε is mainly due to a pronounced increase in the centre of mass of the charge on the electric dipole of the Hb molecule. The results from the absorption spectra indicate that the ratio of met-haemoglobin to oxy-haemoglobin is altered by irradiation. Moreover, the results from the delayed effect studies show that the structure and function of the newly generated Hb molecules are altered and dissimilar to that of healthy Hb.
机译:本研究旨在分析与暴露于50Hz 6kV / m电场,1 mSv的快速中子剂量和混合的大鼠红细胞(rbcs)的血红蛋白(rbcs)的血红蛋白(Hb)暴露相关的辐射风险来自快中子的辐射和分布在电场中的电场在三周内以5天/周和8小时/天的速度分布。对于所有样品,均测量了在1 kHz至5 MHz频率范围内血红蛋白分子的介电常数和吸收光谱。介电弛豫结果表明,来自所有受辐照动物的rbcs的介电增量(Δε)均增加,这表明电偶极子增加。此外,结果表明,被辐照分子的弛豫时间(τ)和分子半径(r)减小,这表明Δε的增加主要归因于表面上电荷质心的明显增加。血红蛋白分子的电偶极子。吸收光谱的结果表明,通过辐射改变了血红蛋白与氧血红蛋白的比例。此外,延迟效应研究的结果表明,新生成的血红蛋白分子的结构和功能发生了变化,与健康血红蛋白的分子结构和功能不同。

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