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首页> 外文期刊>Journal of enzyme inhibition and medicinal chemistry. >Biochemical characterization of the chloroplastic β -carbonic anhydrase from Flaveria bidentis (L.) “Kuntze”
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Biochemical characterization of the chloroplastic β -carbonic anhydrase from Flaveria bidentis (L.) “Kuntze”

机译：黄酮（L。）“ Kuntze”的叶绿体β-碳酸酐酶的生化特性

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C3 and C4 plant carbonic anhydrases (CAs) are zinc-enzymes that catalyze the reversible hydration of CO2. They are sub-divided in three classes: α, β and γ, being distributed between both photosynthetic subtypes. The C4 dicotyledon species Flaveria bidentis (L.) “Kuntze” contains a small gene family encoding three distinct β-CAs, named FbiCA1, FbiCA2 and FbiCA3. We have expressed and purified recombinant FbiCA1, which is localized in the chloroplast where it is thought to play a role in lipid biosynthesis and antioxidant activity, and biochemically characterized it by spectroscopic and inhibition experiments. FbiCA1 is a compact octameric protein that is moderately inhibited by carboxylate molecules. Surprisingly, pyruvate, but not lactate, did not inhibit FbiCA1 at concentrations up to 10?mM, suggesting that its capacity to tolerate high pyruvate concentration reflects the high concentration of pyruvate in the chloroplasts of bundle-sheath and mesophyll cells involved in C4 photosynthesis.
机译：C 3 和C 4 植物碳酸酐酶（CAs）是催化CO 2 可逆水合作用的锌酶。它们分为三类：α，β和γ，分布在两种光合亚型之间。 C 4 双子叶植物物种Flaveria bidentis（L。）“ Kuntze”包含一个小的基因家族，其编码三个不同的β-CA，分别命名为FbiCA1，FbiCA2和FbiCA3。我们已经表达和纯化了重组FbiCA1，它位于叶绿体中，据认为在脂质生物合成和抗氧化活性中起作用，并通过光谱和抑制实验对其进行了生化表征。 FbiCA1是一种紧凑的八聚体蛋白，可被羧酸盐分子适度抑制。令人惊讶的是，丙酮酸而不是乳酸在浓度高达10？mM时并未抑制FbiCA1，这表明其耐受高丙酮酸浓度的能力反映了参与C sub的束鞘和叶肉细胞叶绿体中丙酮酸的高浓度。 > 4 光合作用。

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