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首页> 外文期刊>Dementia and Geriatric Cognitive Disorders Extra >Long-Term Storage Effects on Stability of Aβ1–40, Aβ1–42, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays
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Long-Term Storage Effects on Stability of Aβ1–40, Aβ1–42, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays

机译：长期储存对人体血浆样品中Aβ1–40，Aβ1–42和总Tau蛋白稳定性的影响（通过免疫磁还原法测定）

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Background: The stability of Alzheimer’s disease (AD) biomarkers in plasma, measured by immunomagnetic reduction (IMR) after long-term storage at –80°C, has not been established before. Method: Ninety-nine human plasma samples from 53 normal controls (NCs), 5 patients with amnestic mild cognitive impairment (aMCI), and 41 AD patients were collected. Each plasma sample was aliquoted and stored as single-use aliquots at –80°C. The baseline measurements for Aβ1–40, Aβ1–42, and total Tau protein (T-Tau) concentrations for each sample were done within 3 months of blood draw by IMR. They are referred to as baseline concentrations. A separate aliquot from each sample was assayed with IMR to assess the stability of the measured analytes during storage at –80°C between 1.1 and 5.4 years. This is referred to as a repeated result. Results: IMR shows that plasma levels of Aβ1–40 and Aβ1–42 exhibit stability over 5-year storage at –80°C and that plasma levels of T-Tau are less stable (approximately 1.5 years). Conclusion: Although the measured concentrations of T-Tau in human plasma may alter during storage, the diagnostic utility of the results are only slightly affected when the product of Aβ1–42 and T-Tau concentrations are used. The results show that the overall agreement between baseline and repeated measurements in the ability of discriminating NCs from aMCI/AD patients is higher than 80%.
机译：背景：长期未于–80°C储存后，通过免疫磁还原（IMR）测量的阿尔茨海默氏病（AD）生物标志物在血浆中的稳定性。方法：收集来自53名正常对照（NC），5名轻度轻度认知障碍（aMCI）的患者和41名AD患者的99份人体血浆样品。将每个血浆样品等分并作为一次性使用的等分试样保存在–80°C下。通过IMR在抽血的3个月内对每个样品的Aβ1–40，Aβ1–42和总Tau蛋白（T-Tau）浓度进行基线测量。它们被称为基线浓度。使用IMR对每个样品的单独等分试样进行分析，以评估在–80°C下储存1.1至5.4年期间所测分析物的稳定性。这被称为重复结果。结果：IMR显示，血浆Aβ1–40和Aβ1–42在–80°C下可保存5年，具有稳定性，而T-Tau的血浆水平不稳定（约1.5年）。结论：尽管在储存过程中人体血浆中T-Tau的测定浓度可能会发生变化，但当使用Aβ1-42和T-Tau浓度的乘积时，对结果的诊断效用仅受到轻微影响。结果表明，基线和重复测量之间在区分aMCI / AD患者NCs的能力方面的总体一致性高于80％。

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