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首页> 外文期刊>Journal of Epilepsy and Clinical Neurophysiology >Hippocampal proteomic profile in temporal lobe epilepsy
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Hippocampal proteomic profile in temporal lobe epilepsy

机译：颞叶癫痫的海马蛋白质组学特征

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In this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control. Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy.
机译：在这项研究中，我们使用蛋白质组学方法获得了人类癫痫海马的蛋白质谱（N = 6）并控制了从尸检中获得的对照海马（N = 6）。我们使用二维凝胶电泳（2-D）结合HPLC和质谱（MALDI-TOF）来鉴定差异表达的蛋白质。比较患者海马和对照组海马，差异表达了九种蛋白质。 TLE患者海马中增加的蛋白质包括：血清白蛋白的同工型1，HSP 70，与二氢嘧啶酶相关的蛋白2，髓鞘碱性蛋白的同工型1，质子ATPase催化亚基A和丙酮酸脱氢酶的二氢脂酰赖氨酸残基乙酰转移酶组分复杂。血影蛋白α链（fodrin）的亚型3的表达下调，而仅在TLE患者的海马中检测到谷胱甘肽S-转移酶P和DJ-1（PARK7）蛋白。综上所述，我们的结果提供了证据，证明与TLE病理生理相关的代谢紊乱与氧化损伤之间存在直接联系。此外，指示生物标记物作为针对癫痫的疗法有待进一步研究。

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