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首页> 外文期刊>Journal of Epithelial Biology & Pharmacology >Gradient Perfusion Culture - Simulating a Tissue-Specific Environment for Epithelia in Biomedicine
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Gradient Perfusion Culture - Simulating a Tissue-Specific Environment for Epithelia in Biomedicine

机译：梯度灌注培养-在生物医学中模拟上皮组织的特定环境

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摘要

Epithelia act in the organism as biological barriers. All of them are exposed to different environments at theluminal and basal side. To simulate such a tissue-specific situation Minusheet? gradient perfusion culture was developed.For pharmaceutical research, biomaterial testing and tissue engineering epithelial cells are cultured on individually selectedsupports (1). Growing epithelia are stabilized within a tissue carrier (2). Long term culture is performed in a gradientperfusion container (3). To expose epithelia to a tissue-specific environment fresh media of different composition aretransported parallel to the luminal and basal compartment of the gradient container. During culture leakage, edge damageand pressure differences have to be avoided. Harvest of intact epithelia is promoted by the use of biocompatible supportsand innovative equipment such as transport of oxygen-rich and gas bubble-free medium. Actual literature demonstratesthat gradient perfusion culture is an effective method to investigate barrier functions under realistic conditions. Examplesof application comprise renal epithelia, retina, blood-air barrier, blood-brain barrier including aspects of tissue-specificdevelopment and regeneration.
机译：上皮细胞在生物体内起着生物屏障的作用。它们都在管腔和基底侧暴露于不同的环境。要模拟这样的组织特定情况Minusheet？开发了梯度灌注培养法，在药物研究，生物材料测试和组织工程上皮细胞培养于单独选择的支持物上（1）。生长中的上皮细胞在组织载体（2）内稳定。在梯度灌注容器（3）中进行长期培养。为了使上皮暴露于组织特异性的环境，将不同组成的新鲜培养基平行于梯度容器的腔和基底腔室输送。在培养液泄漏期间，必须避免边缘损坏和压力差。通过使用生物相容性支持物和创新设备（例如运输富氧和无气泡的培养基），可以促进完整上皮的收获。实际文献表明，梯度灌流培养是研究现实条件下屏障功能的有效方法。应用的实例包括肾上皮，视网膜，血气屏障，血脑屏障，包括组织特异性发育和再生的方面。

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