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首页> 外文期刊>Journal of genetics >Adenosine monophosphate-activated protein kinase from the mud crab, Scylla paramamosain: cDNA cloning and profiles under cold stress
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Adenosine monophosphate-activated protein kinase from the mud crab, Scylla paramamosain: cDNA cloning and profiles under cold stress

机译：泥蟹Scyla paramamosain的腺苷单磷酸激活蛋白激酶：低温胁迫下的cDNA克隆和概况

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摘要

Adenosine monophosphate-activated protein kinase (AMPK), an important energy sensor, is crucial for organism survival under adverse conditions. In this study, the roles of this gene under cold stress in a warm-water mud crab, Scylla paramamosain was investigated. The full-length cDNA (SpAMPK) was 1884 bp and its open reading frame of 1566 bp was isolated and characterized. The expressions of SpAMPK detected by quantitative real-time PCR (qRT-PCR) in various tissues revealed that the highest expression was in the hepatopancreas. The profiles of SpAMPK gene in the hepatopancreas, chela muscleand gill were detected when the subadult crabs were exposed to the four temperature conditions of 10, 15, 20 and 25a—|C. The results showed that the expression patterns of SpAMPK mRNA in the three tissues were significantly higher when crabs were exposed to 15a—|C than the other three temperature treatments, while at 10a—|C treatment, the SpAMPK mRNA was lowestamong the four temperature treatments. These findings suggested that the high expression of SpAMPK mRNA might initiate ATP-producing pathways to generate energy to cope with cold stress at 15a—|C treatment, which was slightly below the range of optimum temperatures; while treatment at 10a—|C, far lower than optima, the low expression of SpAMPK mRNA could reduce the energy expenditure and thus induce the crabs into cold anesthesia. The results of SpAMPK in this study might contribute to the understanding of the molecular mechanism of acclimation to cold hardiness in S. paramamosain.
机译：腺苷单磷酸激活蛋白激酶（AMPK）是一种重要的能量传感器，对于不利条件下的生物存活至关重要。在这项研究中，研究了该基因在温水泥蟹Scylla paramamosain中在冷胁迫下的作用。全长cDNA（SpAMPK）为1884 bp，并对其开放阅读框1566 bp进行了分离和鉴定。通过定量实时PCR（qRT-PCR）检测到的SpAMPK在各种组织中的表达表明最高的表达是在肝胰腺中。当亚成年蟹暴露于10、15、20和25a-C的四个温度条件下时，在肝胰腺，螯肌和腮中检测到SpAMPK基因的概况。结果显示，当螃蟹暴露于15a- | C时，三种组织中SpAMPK mRNA的表达模式明显高于其他三种温度处理，而在10a- | C处理下，蟹中SpAMPK mRNA的表达在四种温度处理中最低。 。这些发现表明，SpAMPK mRNA的高表达可能会启动ATP产生途径，以产生能量来应对15a- | C处理下的冷胁迫，该温度略低于最佳温度范围。在10a- | C处理时（远低于最佳值），SpAMPK mRNA的低表达可以减少能量消耗，从而诱导螃蟹进行冷麻醉。 SpAMPK在这项研究中的结果可能有助于理解副嗜珠菌中抗寒性的分子机制。

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