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首页> 外文期刊>Journal of genetics >Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with a€?floral bud distortiona€? in soybean
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Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with a€?floral bud distortiona€? in soybean

机译：花器官的细胞学行为和计算机表达与花芽畸变有关的差异表达转录本片段的计算机表征在大豆中

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An attempt was made to understand the a€?floral bud distortiona€? (FBD), an unexplored disorder prevailing in soybean. Cytological behaviour of floral reproductive organs and in silico characterization of differentially expressed transcript-derivedfragments (TDFs) in symptomatic and asymptomatic soybean plants were carried out. Pollens in asymptomatic plants do not have defects in number, size, shape and function. However, in symptomatic plant, pollens were found nonviable, abnormalin shape and with reduced germination ability. Here, we employed a computational approach, exploring invaluable resources. The tissue-specific transcript profile of symptomatic and asymptomatic sources was compared to determine differentiallyexpressed TDFs associated with FBD to improve its basic understanding. A total of 60 decamer primers produced 197 scorable amplicons, ranged 162a€“1130 bp, of which 171 were monomorphic and 26 were differentially regulated. Reproducible TDFs were sequenced and characterized for their homology analysis, annotation, proteina€“protein interaction, subcellular localization and their physical mapping. Homology-based annotation of TDFs in soybean revealed presence of two characterized and seven uncharacterized hits. Annotation of characterized sequences showed presence of genes, namely auxin response factor 9(ARF9) and forkhead-associated (FHA) domain, which are directly involved in plant development through various pathways, such as hormonal regulation, plant morphology, embryogenesis and DNA repair.
机译：试图理解“花芽畸变”。 （FBD），一种在大豆中普遍存在的未开发的疾病。进行了有症状和无症状大豆植物中花生殖器官的细胞学行为和计算机表达差异表达的转录片段（TDFs）的计算机表征。无症状植物中的花粉在数量，大小，形状和功能上均没有缺陷。然而，在有症状的植物中，发现花粉不可行，形状异常且发芽能力降低。在这里，我们采用了一种计算方法，探索了宝贵的资源。比较有症状和无症状来源的组织特异性转录本谱，以确定与FBD相关的差异表达TDF，以提高其基本理解。总共60个decamer引物产生197个可评分的扩增子，范围为162a-1130 bp，其中171个是单态性，而26个受到差异调节。对可重现的TDF进行了测序，并对其同源性分析，注释，蛋白，蛋白相互作用，亚细胞定位及其物理定位进行了表征。大豆中TDF的基于同源性的注释显示存在两个特征性和七个非特征性命中。特征序列的注释显示存在基因，即生长素应答因子9（ARF9）和叉头相关（FHA）结构域，它们通过多种途径直接参与植物发育，例如激素调节，植物形态学，胚胎发生和DNA修复。

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