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首页> 外文期刊>Journal of genetics >Marker-assisted improvement of the elite restorer line of rice, RPHR-1005 for resistance against bacterial blight and blast diseases
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Marker-assisted improvement of the elite restorer line of rice, RPHR-1005 for resistance against bacterial blight and blast diseases

机译：标记的水稻优良恢复系RPHR-1005的抗细菌白叶枯病和稻瘟病抗性改良

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This study was carried out to improve the RPHR-1005, a stable restorer line of the popular medium slender grain type rice hybrid, DRRH-3 for bacterial blight (BB) and blast resistance through marker-assisted backcross breeding (MABB). Twomajor BB resistance genes, Xa21 and Xa33 and a major blast resistance gene, Pi2 were transferred to RPHR-1005 as two individual crosses. Foreground selection for Xa21, Xa33, Pi2, Rf3 and Rf4 was done by using gene-specific functional markers, while 59 simple sequence repeat (SSR) markers polymorphic between the donors and recipient parents were used to select the best plant possessing target resistance genes at each backcross generation. Backcrossing was continued till BCa??Fa?? and a promising homozygous backcross derived line possessing Xa21 + Pi2 and another possessing Xa33 were intercrossed to stack the target resistance genes into the genetic background of RPHR-1005. At ICF4, 10 promising lines possessing three resistance genes in homozygous condition along with fine-grain type, complete fertility restoration, better panicle exertion and taller plant type (compared to RPHR-1005) were identified.
机译：进行这项研究的目的是通过标记辅助回交育种（MABB）改良RPHR-1005，这是一种流行的中等细长粒型水稻杂交种的稳定恢复系DRRH-3，用于细菌白叶枯病（BB）和抗瘟性。将两个主要的BB抗性基因Xa21和Xa33和一个主要的抗稻瘟性基因Pi2作为两个单独的杂交转移到RPHR-1005。 Xa21，Xa33，Pi2，Rf3和Rf4的前景选择是通过使用基因特异性功能标记完成的，而供体和受体亲本之间多态性的59个简单序列重复（SSR）标记被用于选择具有目标抗性基因的最佳植物。每个回交代。继续进行回交直到BCa ?? Fa ??。将具有Xa21 + Pi2和另一个具有Xa33的有希望的纯合回交衍生品系进行杂交，以将目标抗性基因堆叠到RPHR-1005的遗传背景中。在ICF4上，鉴定了10个有希望的品系，这些品系在纯合条件下具有三个抗性基因，以及细粒型，完整的育性恢复，更好的穗活力和更高的植物型（与RPHR-1005比较）。

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