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首页> 外文期刊>Journal of genetics >Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum
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Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum

机译：Wami罗非鱼，Oreochromis urolepis hornorum中与性别相关的微卫星标记的筛选和鉴定

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In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated((P0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assistedselection in tilapia breeding programmes to produce monosex male tilapia
机译：在这项研究中，选择了15个与罗非鱼性别确定有关的微卫星标记的引物对，并在Wami罗非鱼，Oreochromis urolepis hornorum中扩增。连锁群3（LG3）上的一种标记物UNH168与表型性别相关（（P <0.001）），但在两种性别中均检测到9个基因型，在女性样本中仅检测到99 bp等位基因，而在男性和女性样本中均存在141、149和157-bp等位基因，UNH168通过荧光原位杂交（FISH）定位在最大的罗非鱼染色体对的长臂上（染色体1，相当于LG3）。连锁的微卫星标记可能在罗非鱼育种计划中用于标记辅助选择，以产生单性雄性罗非鱼

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