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Molecular diversity and genetic relationships in Secale

机译:Secale中的分子多样性和遗传关系

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The objective of this study was to quantify the molecular diversity and to determine the genetic relationships amongSecalespp. and among cultivars ofSecale cerealeusing RAPDs, ISSRs and sequence analysis of six exons ofScMATE1gene.Thirteen ryes (cultivated and wild) were genotyped using 21 RAPD and 16 ISSR primers. A total of 435 markers (242 RAPDsand 193 ISSRs) were obtained, with 293 being polymorphic (146 RAPDs and 147 ISSRs). Two RAPD and nine ISSR primersgenerated more than 80% of polymorphism. The ISSR markers were more polymorphic and informative than RAPDs. Further,69% of the ISSR primers selected achieved at least 70% of DNA polymorphism. The study of six exons of theScMATE1gene also demonstrated a high genetic variability that subsists inSecalegenus. One difference observed in exon 1 sequencesfromS. vaviloviiseems to be correlated with Al sensitivity in this species. The genetic relationships obtained using RAPDs,ISSRs and exons ofScMATE1gene were similar.S. ancestrale,S. kuprijanoviiandS. cerealewere grouped in the same clusterandS. segetalewas in another cluster.S. vaviloviishowed evidences of not being clearly an isolate species and having greatintraspecific difference
机译:这项研究的目的是量化分子多样性并确定Secalespp之间的遗传关系。在使用RAPD进行的谷类谷物栽培品种中,对ISSR进行了分析,并对六个ScMATE1基因外显子进行了序列分析。使用21种RAPD和16种ISSR引物对13个黑麦(栽培和野生)进行了基因分型。总共获得了435个标记(242个RAPD和193个ISSR),其中293个是多态的(146个RAPD和147个ISSR)。两个RAPD和九个ISSR引物产生了80%以上的多态性。 ISSR标记比RAPD具有更多的多态性和信息性。此外,选择的69%的ISSR引物实现了至少70%的DNA多态性。对ScMATE1基因的六个外显子的研究还表明,存在于Secalegenus中的遗传变异性很高。在S的外显子1序列中观察到一个差异。 vavilovii似乎与该物种的铝敏感性相关。利用RAPD,ISSR和ScMATE1基因的外显子获得的遗传关系相似。祖斯特拉尔kuprijanoviiandS。谷类食物分组在同一集群中。 segetale位于另一个集群中。 vavilovii显示的证据不是明显的分离物种,种内差异很大

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