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首页> 外文期刊>Journal of genetics >Molecular cloning, characterization and expression of WAG-2 alternative splicing transcripts in developing spikes of Aegilops tauschii
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Molecular cloning, characterization and expression of WAG-2 alternative splicing transcripts in developing spikes of Aegilops tauschii

机译：WAG-2选择性剪接转录本在节节蓬中生长穗的分子克隆，表征及表达

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摘要

WAG-2 is a C-class MADS-box gene, which is orthologous to AGAMOUS (AG )inArabidopsis. The AG group C-classMADS-box genes are involved in stamen and pistil identity. In this study, two WAG-2 transcripts, namely, WAG-2f and WAG-2g, were isolated and characterized from Aegilops tauschii . The open reading frames of WAG-2f and WAG-2g were 825 and 822 bp, respectively, encoding 275 and 274 amino acid residues. BLAST searches of partial WAG-2 genomic sequence againstthe draft sequence of Ae. tauschii genome database revealed the complex structure of WAG-2 gene, which consisted of seven exons and six introns. TheWAG-2f and WAG-2g cDNAs were two alternative splicing transcripts. The alternative splicing events were produced by an alternative 5 ' splice site. The expression level of WAG-2f transcript, which was extremely weak inyoung spikes of floret primordium formation stage, increased as the spikes developed. The highest expression was observed in the spikes at the anther separation stage. Low expression levels of WAG-2f were also detected at the tetrad stage. The WAG-2g transcript was expressed at all four stages of spike development but at a relatively low level. The expression pattern of thetwo transcripts was distinctly different during floral development, thereby suggesting a functional divergence.
机译：WAG-2是C类MADS-box基因，与拟南芥中的AGAMOUS（AG）同源。 AG组的C-classMADS-box基因与雄蕊和雌蕊的身份有关。在这项研究中，分离了两个WAG-2转录本，即WAG-2f和WAG-2g，并对其进行了鉴定。 WAG-2f和WAG-2g的开放阅读框分别为825和822 bp，编码275和274个氨基酸残基。针对Ae的拔模序列进行部分WAG-2基因组序列的BLAST搜索。 tauschii基因组数据库揭示了WAG-2基因的复杂结构，该基因由七个外显子和六个内含子组成。 WAG-2f和WAG-2g cDNA是两个可选的剪接转录本。替代的剪接事件由替代的5'剪接位点产生。 WAG-2f转录物的表达水平随着小穗的形成而增加，这是小花原基形成期的年轻幼穗。在花药分离阶段的穗中观察到最高的表达。在第四阶段也检测到了低表达的WAG-2f。 WAG-2g转录本在穗发育的所有四个阶段表达，但水平较低。两种转录本的表达模式在花发育过程中明显不同，从而表明功能上的差异。

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