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首页> 外文期刊>Journal of genetics >Molecular mapping of a stripe rust resistance gene in wheat line C51
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Molecular mapping of a stripe rust resistance gene in wheat line C51

机译：小麦C51品系抗条锈基因的分子定位

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Stripe rust, a major disease in areas where cool temperatures prevail, can strongly influence grain yield. To control this disease, breeders have incorporated seedling resistance genes from a variety of sources outside the primary wheat gene pool. The wheat line C51, introduced from the International Center for Agricultural Research in the Dry Areas (ICARDA), Syria, confers resistance to all races of Puccinia striiformis f. sp. tritici (PST) in China. To map the resistant gene(s) against stripe rust in wheat line C51, 212 F8 recombinant inbred lines (RILs) derived from the cross X440 ?— C51 were inoculated with Chinese PST race CYR33 (Chinese yellow rust, CYR) in the greenhouse. The result showed that C51 carried a single dominant gene for resistance (designated YrC51) to CYR33. Simple sequence repeat (SSR) and resistance gene-analogue polymorphism (RGAP) markers that were polymorphic between the parents were used for genotyping the 212 F8 RILs. YrC51 was closely linked to two SSR loci on chromosome 2BS with genetic distances of 5.1 cM (Xgwm429) and 7.2 cM (Xwmc770), and to three RGAP markers C51R1 (XLRR For / NLRR For), C51R2 (CLRR Rev / Cre3LR-F) and C51R3 (Pto kin4/ NLRRINV2) with genetic distances of 5.6, 1.6 and 9.2 cM, respectively. These RGAP-linked markers were then converted into STS markers.Among them, one STS marker, C51STS-4, was located at a genetic distance of 1.4 cM to YrC51 and was closely associated with resistance when validated in several populations derived from crosses between C51 and Sichuan cultivars. The results indicated that C51STS-4 can be used for marker assisted selection (MAS) and would facilitate the pyramiding of YrC51 with other genes for stripe rust resistance.
机译：条锈病是低温地区的主要疾病，会严重影响谷物的产量。为了控制这种疾病，育种者已将小麦抗性基因整合到了小麦原始基因库之外的多种来源中。从叙利亚干旱地区国际农业研究中心（ICARDA）引进的小麦品系C51，赋予了对小麦条锈菌所有种族的抵抗力。 sp。中国的小麦（PST）。为了在小麦品系C51中绘制抗条锈病的基因图谱，在温室中用中国PST种CYR33（中国黄锈，CYR）接种了来自X440？C51杂交的212个F8重组自交系（RIL）。结果显示C51携带单一的显性基因（称为YrC51）对CYR33的抗性。亲本之间多态的简单序列重复（SSR）和抗性基因类似物多态性（RGAP）标记用于212 F8 RIL的基因分型。 YrC51与2BS染色体上两个遗传距离分别为5.1 cM（Xgwm429）和7.2 cM（Xwmc770）的SSR基因座以及三个RGAP标记C51R1（XLRR For / NLRR For），C51R2（CLRR Rev / Cre3LR-F）紧密相连。和C51R3（Pto kin4 / NLRRINV2）的遗传距离分别为5.6、1.6和9.2 cM。然后将这些与RGAP相关的标记转化为STS标记，其中一个STS标记C51STS-4位于与YrC51的遗传距离为1.4 cM的位置，并且在C51之间的杂交得到的几个种群中进行验证时与耐药性密切相关和四川品种。结果表明，C51STS-4可用于标记辅助选择（MAS），并有助于YrC51与其他抗条纹锈病基因的金字塔。

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