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首页> 外文期刊>Journal of genetics >Identification and characterization of the RCI2 gene family in maize (Zea mays)
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Identification and characterization of the RCI2 gene family in maize (Zea mays)

机译：玉米（Zea mays）RCI2基因家族的鉴定与表征

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Rare-cold-inducible (RCI2) genes are structurally conserved members that encode small, highly hydrophobic proteins involved in response to various abiotic stresses. Phylogenetic and functional analyses of these genes have been conducted in Arabidopsis, but an extensive investigation of the RCI2 gene family has not yet been carried out in maize. In the present study, 10 RCI2 genes were identified in a fully sequenced maize genome. Structural characterization and expression pattern analysis of 10 ZmRCI2s (Zea mays RCI2 genes) were subsequently determined. Sequence and phylogenetic analyses indicated that ZmRCI2s are highly conserved, and most of them could be grouped with their orthologues from other organisms. Chromosomal location analysis indicated that ZmRCI2s were distributed unevenly on seven chromosomes with two segmental duplication events, suggesting that maize RCI2 gene family is an evolutionarily conserved family. Putative stress-responsive cis-elements were detected in the 2-kb promoter regions of the 10 ZmRCI2s. In addition, the 10 ZmRCI2s showed different expression patterns in maize development based on transcriptome analysis. Further, microarray and quantitative real-time PCR (qRT-PCR) analysis showed that each maize RCI2 genes were responsive to drought stress, suggesting their important roles in drought stress response. The results of this work provide a basis for future cloning and application studies of maize RCI2 genes.
机译：罕见冷诱导（RCI2）基因是结构保守的成员，其编码参与各种非生物胁迫反应的小而高度疏水的蛋白质。这些基因的系统发生和功能分析已在拟南芥中进行，但尚未在玉米中对RCI2基因家族进行广泛研究。在本研究中，在完全测序的玉米基因组中鉴定出10个RCI2基因。随后确定了10个ZmRCI2（Zea mays RCI2基因）的结构特征和表达模式分析。序列和系统发育分析表明，ZmRCI2s是高度保守的，并且大多数可以与来自其他生物的直向同源物分组。染色体定位分析表明ZmRCI2s在7条染色体上分布不均，具有两个节段性重复事件，表明玉米RCI2基因家族是一个进化保守的家族。在10个ZmRCI2的2-kb启动子区域中检测到推定的应激反应顺式元件。另外，基于转录组分析，这10个ZmRCI2在玉米发育中显示出不同的表达模式。此外，微阵列和定量实时PCR（qRT-PCR）分析表明，每个玉米RCI2基因对干旱胁迫都有反应，表明它们在干旱胁迫反应中的重要作用。这项工作的结果为今后玉米RCI2基因的克隆和应用研究提供了基础。

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