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首页> 外文期刊>Journal of genetics >Construction of microsatellite-based linkage map and mapping of nectarilessness and hairiness genes in Gossypium tomentosum
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Construction of microsatellite-based linkage map and mapping of nectarilessness and hairiness genes in Gossypium tomentosum

机译：基于微卫星的连锁图谱的构建及棉铃虫油桃和毛羽基因的定位

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Gossypium tomentosum, a wild tetraploid cotton species with AD genomes, possesses genes conferring strong fibers and high heat tolerance. To effectively transfer these genes into Gossypium hirsutum, an entire microsatellite (simple sequence repeat, SSR)-based genetic map was constructed using the interspecific cross of G. hirsutum ?— G. tomentosum (HT). We detected 1800 loci from 1347 pairs of polymorphic primers. Of these, 1204 loci were grouped into 35 linkage groups at LOD a‰￥4. The map covers 3320.8 cM, with a mean density of 2.76 cM per locus. We detected 420 common loci (186 in the At subgenome and 234 in Dt) between the HT map and the map of TM-1 (G. hirsutum) and Hai 7124 (G. barbadense; HB map). The linkage groups were assigned chromosome numbers based on location of common loci and the HB map as reference. A comparison of common markers revealed that no significant chromosomal rearrangement exist between G. tomentosum and G. barbadense. Interestingly, however, we detected numerous (33.7%) segregation loci deviating from 3:1 ratio ($P lt 0.05$) in HT, mostly clustering on eight chromosomes in the Dt subgenome, with some on three chromosomes in At. Two morphological traits, leaf hairiness and leaf nectarilessness were mapped on chromosomes 6 (A6) and 26 (D12), respectively. The SSR-based map constructed in this study will be useful for further genetic studies on cotton breeding, including mapping loci controlling quantitative traits associated with fiber quality, stress tolerance and developing chromosome segment specific introgression lines from G. tomentosum into G. hirsutum using marker-assisted selection.
机译：棉铃虫（Gossypium tomentosum）是具有AD基因组的野生四倍体棉种，具有赋予强纤维和高耐热性的基因。为了将这些基因有效地转移到陆地棉中，使用陆地棉γ-绒毛甘蓝（HT）的种间杂交构建了一个完整的基于微卫星（简单序列重复，SSR）的遗传图谱。我们从1347对多态性引物中检测到1800个基因座。其中1204个基因座按LOD≥4分为35个连锁组。该图覆盖3320.8 cM，每个位点的平均密度为2.76 cM。我们在HT图谱与TM-1（G. hirsutum）图谱和Hai 7124（G. barbadense; HB图谱）图谱之间检测到420个共同基因位点（At亚基因组186个，Dt 234个）。根据常见基因座的位置和HB图谱为连锁组分配染色体编号。常用标记物的比较显示，绒毛甘蓝和巴巴登巴霉之间不存在明显的染色体重排。然而，有趣的是，我们在HT中检测到许多偏离（33.7％）分离位点的比例为3：1（$ P lt 0.05 $），主要集中在Dt亚基因组的8条染色体上，有些在At的3条染色体上。分别在6号染色体（A6）和26号染色体（D12）上绘制了叶片毛羽和叶片无核这两个形态特征。在这项研究中构建的基于SSR的图谱将对棉花育种的进一步遗传研究有用，包括对控制与纤维质量，胁迫耐性相关的定量性状的基因座进行绘图，并使用标记物将绒毛甘蓝发育成陆地棉的染色体片段特异性渗入系。辅助选择。

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