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首页> 外文期刊>Journal of genetics >Evaluation of the association of SLC11A1 gene polymorphism with incidence of paratuberculosis in goats
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Evaluation of the association of SLC11A1 gene polymorphism with incidence of paratuberculosis in goats

机译：SLC11A1基因多态性与山羊副结核病发生率的相关性评估

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Paratuberculosis is one of the chronic granulomatous enteritis that predominantly affects ruminantsworldwide, caused by Mycobacterium avium ssp. paratuberculosis (MAP). In ruminants, microsatellite polymorphisms of the 3' untranslated region (3'UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to intracellular pathogen infections. Thisresearch was carried out to detect the polymorphisms in A and B regions of the 3'UTR of SLC11A1 gene and to evaluate the potential association between these polymorphisms and MAP infection in goats. MAP-specific antibodies were detected by ELISA and MAP infection was confirmed by IS900 PCR in 150 adult goats from different regions of Kerala, India. The polymorphism ofmicrosatellite regions A and B at 3'UTR of the SLC11A1 gene was analysed in goats by an automated technique, fragment analysis, using fluorescent-tagged forward primers. Eight alleles with sizes ranging from 221 to 239 bp were found in region A. Region B revealed two alleles, 117 bp (B7) and 119 bp (B8). Animals with B8 alleles were found to have higher incidence of paratuberculosis than animals with B7alleles (P 0.01). There was no statistically significant association found between region A genotypes and paratuberculosis incidence. These results suggest that caprine SLC11A1 gene has significant role in paratuberculosis resistance ingoats and further studies might help in development of a PCR-based genotyping test for paratuberculosis resistance and selection of superior animals for future goat breeding programmes.
机译：副结核是由鸟分枝杆菌属引起的慢性肉芽肿性肠炎之一，主要影响全世界的反刍动物。副结核病（MAP）。在反刍动物中，溶质载体家族11成员A1（SLC11A1）基因的3'非翻译区（3'UTR）的微卫星多态性与对细胞内病原体感染的抵抗力有关。进行了这项研究，以检测SLC11A1基因3'UTR的A和B区的多态性，并评估这些多态性与山羊MAP感染之间的潜在关联。通过ELISA检测到MAP特异性抗体，并通过IS900 PCR在来自印度喀拉拉邦不同地区的150只成年山羊中证实了MAP感染。通过自动化技术，片段分析，使用荧光标记的正向引物，对山羊中SLC11A1基因3'UTR处的微卫星区域A和B的多态性进行了分析。在区域A中发现了8个等位基因，大小在221至239 bp之间。区域B显示了两个等位基因，分别为117 bp（B7）和119 bp（B8）。发现具有B8等位基因的动物比具有B7等位基因的动物的副结核病发生率更高（P <0.01）。在区域A的基因型和肺结核旁发生率之间没有统计学上的显着关联。这些结果表明，山羊SLC11A1基因在抗副结核病的入侵中具有重要作用，进一步的研究可能有助于开发基于PCR的抗副结核病基因分型测试，并为将来的山羊育种计划选择优良的动物。

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