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首页> 外文期刊>Journal of Extracellular Vesicles >Extracellular membrane vesicles from umbilical cord blood-derived MSC protect against ischemic acute kidney injury, a feature that is lost after inflammatory conditioning
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Extracellular membrane vesicles from umbilical cord blood-derived MSC protect against ischemic acute kidney injury, a feature that is lost after inflammatory conditioning

机译：源自脐带血MSC的细胞外膜囊泡可预防缺血性急性肾脏损伤，炎症性调节后该功能丧失

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BackgroundMesenchymal stromal cells (MSC) are shown to have a great therapeutic potential in many immunological disorders. Currently the therapeutic effect of MSCs is considered to be mediated via paracrine interactions with immune cells. Umbilical cord blood is an attractive but still less studied source of MSCs. We investigated the production of extracellular membrane vesicles (MVs) from human umbilical cord blood derived MSCs (hUCBMSC) in the presence (MVstim) or absence (MVctrl) of inflammatory stimulus.MethodshUCBMSCs were cultured in serum free media with or without IFN-γ and MVs were collected from conditioned media by ultracentrifugation. The protein content of MVs were analyzed by mass spectrometry. Hypoxia induced acute kidney injury rat model was used to analyze the in vivo therapeutic potential of MVs and T-cell proliferation and induction of regulatory T cells were analyzed by co-culture assays.ResultsBoth MVstim and MVctrl showed similar T-cell modulation activity in vitro, but only MVctrls ...
机译：背景间充质基质细胞（MSC）已显示在许多免疫疾病中具有巨大的治疗潜力。目前，认为MSC的治疗作用是通过旁分泌与免疫细胞的相互作用来介导的。脐带血是一种有吸引力的MSC，但仍未被研究。我们研究了在有（MVstim）有或无（MVctrl）炎性刺激的情况下，从人脐血来源的MSC（hUCBMSC）产生细胞外膜囊泡（MVs）的方法。通过超速离心从条件培养基中收集MV。通过质谱分析MV的蛋白质含量。低氧诱导的急性肾损伤大鼠模型用于分析MVs和T细胞增殖的体内治疗潜能，并通过共培养分析法分析调节性T细胞的诱导。结果MVstim和MVctrl在体外均表现出相似的T细胞调节活性，但只有MVctrls ...

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