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首页> 外文期刊>Journal of Extracellular Vesicles >Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes
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Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

机译：细胞外小泡亚群中不同的RNA谱：凋亡小体，微泡和外泌体

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IntroductionIn recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are still under intense discussion and investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination.MethodEVs released from three different kinds of cell lines: HMC-1, TF-1 and BV-2 were isolated using two centrifugation-based protocols. In protocol 1, apoptotic bodies were collected at 2,000×g, followed by filtering the supernatant through 0.8 μm pores and pelleting of microvesicles at 12,200×g. In protocol 2, apoptotic bodies and microvesicles were collected together at 16,500×g, followed by filtering of the supernatant through 0.2 μm pores and pelleting of exosomes at 120,000×g. Extracellular vesicles were analyzed by transmission electron microscopy, flow cytometr...
机译：引言近年来，旨在了解外泌体以及其他细胞外囊泡生物学的研究数量呈指数增长。然而，膜囊泡的分类及其分离的适当方案仍在激烈的讨论和研究中。分离囊泡时，至关重要的是使用能够分离的囊泡以避免交叉污染.MethodEV从三种不同细胞系HMC-1，TF-1和BV-2中释放的EV通过两次离心分离-基于协议。在方案1中，以2,000 x g收集凋亡小体，然后通过0.8μm孔过滤上清液，并以12,200 x g沉淀微囊泡。在方案2中，将凋亡小体和微囊泡以16500×g的浓度收集在一起，然后通过0.2μm的孔过滤上清液，并以120,000×g的颗粒体沉淀。细胞外囊泡通过透射电镜，流式细胞仪进行分析。

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